The regulation of glucose transporters (GLUT-1 and GLUT-3), in terms of both mRNA and protein, in human retinal endothelial cells was investigated. The cells responded within 1 h of exposure to 5 mM glucose with an increase in the level of GLUT-3 mRNA that was due to an increase in the transcription of the 4.1 kb mRNA of the gene for GLUT-3. In the absence of glucose, the gene for GLUT-1 was not transcribed but the level of GLUT-3 mRNA was increased in these conditions and this was the result of an increase in the transcription of the 4.1 kb mRNA. The level of GLUT-1 and GLUT-3 mRNA was maximal when the cells were exposed to 15 mM glucose. These results are discussed in the light of the glucose regulatory potential of the retinal microvasculature and the implications that this may have for the mechanisms of diabetic retinopathy.
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August 1996
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Research Article|
August 15 1996
Regulation of glucose transporters (GLUT-1 and GLUT-3) in human retinal endothelial cells
Rachel M KNOTT;
Rachel M KNOTT
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*To whom correspondence should be addressed.
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Marie ROBERTSON;
Marie ROBERTSON
1Department of Ophthalmology, University Medical Buildings, Foresterhill, Aberdeen AB9 2ZD, U.K.
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Elizabeth MUCKERSIE;
Elizabeth MUCKERSIE
1Department of Ophthalmology, University Medical Buildings, Foresterhill, Aberdeen AB9 2ZD, U.K.
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John V FORRESTER
John V FORRESTER
1Department of Ophthalmology, University Medical Buildings, Foresterhill, Aberdeen AB9 2ZD, U.K.
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Publisher: Portland Press Ltd
Received:
December 20 1995
Revision Received:
May 02 1996
Accepted:
May 13 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 318 (1): 313–317.
Article history
Received:
December 20 1995
Revision Received:
May 02 1996
Accepted:
May 13 1996
Citation
Rachel M KNOTT, Marie ROBERTSON, Elizabeth MUCKERSIE, John V FORRESTER; Regulation of glucose transporters (GLUT-1 and GLUT-3) in human retinal endothelial cells. Biochem J 15 August 1996; 318 (1): 313–317. doi: https://doi.org/10.1042/bj3180313
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