By using a combination of the methods of reverse transcription-PCR and rapid amplification of cDNA ends, a cDNA for rat pS2 peptide (rpS2) was successfully cloned and sequenced from rat stomach. By RNA blot analysis, the gene was shown to be expressed abundantly in the stomach and only faintly in the duodenum, but not in other tissues including the distal small and large intestines. rpS2 expression was also examined in the rectum during the course of acetic acid-induced colitis; rpS2 mRNA was detected during the acute phase of colitis but not in normal controls or during the recovery phase. On the other hand, expression of rat intestinal trefoil factor (rITF) was down-regulated during the acute phase of colitis and then up-regulated during the recovery phase, whereas rat spasmolytic peptide was not detectable throughout the course of the induced colitis. These results indicate that the patterns and timing of the expression of these trefoil peptides are different from each other. rpS2 may play an important role in the proliferation of intestinal epithelial cells during the acute phase of mucosal ulceration, whereas rITF may be involved in differentiation of the cells, particularly to form goblet cells, during the recovery phase.
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September 1996
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Research Article|
September 15 1996
cDNA cloning of rat pS2 peptide and expression of trefoil peptides in acetic acid-induced colitis
Hiroshi ITOH;
Hiroshi ITOH
*Department of Parasitology, Miyazaki Medical College, Miyazaki 889-16, Japan
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Masaki TOMITA;
Masaki TOMITA
*Department of Parasitology, Miyazaki Medical College, Miyazaki 889-16, Japan
†2nd Department of Surgery, Miyazaki Medical College, Miyazaki 889-16, Japan
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Hirofumi UCHINO;
Hirofumi UCHINO
†2nd Department of Surgery, Miyazaki Medical College, Miyazaki 889-16, Japan
‡2nd Department of Pathology, Miyazaki Medical College, Miyazaki 889-16, Japan
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Takahiko KOBAYASHI;
Takahiko KOBAYASHI
*Department of Parasitology, Miyazaki Medical College, Miyazaki 889-16, Japan
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Hiroaki KATAOKA;
Hiroaki KATAOKA
‡2nd Department of Pathology, Miyazaki Medical College, Miyazaki 889-16, Japan
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Ryo SEKIYA;
Ryo SEKIYA
†2nd Department of Surgery, Miyazaki Medical College, Miyazaki 889-16, Japan
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Yukifumi NAWA
Yukifumi NAWA
§
*Department of Parasitology, Miyazaki Medical College, Miyazaki 889-16, Japan
§To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
April 15 1996
Revision Received:
May 31 1996
Accepted:
June 07 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1996
1996
Biochem J (1996) 318 (3): 939–944.
Article history
Received:
April 15 1996
Revision Received:
May 31 1996
Accepted:
June 07 1996
Citation
Hiroshi ITOH, Masaki TOMITA, Hirofumi UCHINO, Takahiko KOBAYASHI, Hiroaki KATAOKA, Ryo SEKIYA, Yukifumi NAWA; cDNA cloning of rat pS2 peptide and expression of trefoil peptides in acetic acid-induced colitis. Biochem J 15 September 1996; 318 (3): 939–944. doi: https://doi.org/10.1042/bj3180939
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