Fetal rat brown adipocytes show high-affinity binding sites for both insulin-like growth factor I (IGF-I) and insulin. Cell culture for 24 h in the presence of IGF-I or insulin, independently, up-regulated the mRNA expression of adipogenic-related genes, such as fatty acid synthase (FAS), glycerol-3-phosphate dehydrogenase and insulin-regulated glucose transporter Glut4, and down-regulated the expression of phosphoenolpyruvate carboxykinase mRNA in a dose-dependent manner. Moreover, both IGF-I and insulin increased the FAS gene transcription rate at 2 h, producing a time-dependent accumulation of FAS mRNA. Furthermore IGF-I or insulin increased glucose uptake and lipid content throughout the 24 h culture period. Our results suggest that both IGF-I and insulin are major signals involved in initiating and/or maintaining the expression of adipogenic-related genes in fetal rat brown adipocytes.
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October 1996
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Research Article|
October 15 1996
Insulin-like growth factor I and insulin induce adipogenic-related gene expression in fetal brown adipocyte primary cultures
Teresa TERUEL;
Teresa TERUEL
1Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Universidad Complutense, 28040-Madrid, Spain
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Angela M VALVERDE;
Angela M VALVERDE
1Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Universidad Complutense, 28040-Madrid, Spain
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Manuel BENITO;
Manuel BENITO
1Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Universidad Complutense, 28040-Madrid, Spain
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Margarita LORENZO
Margarita LORENZO
*
1Departamento de Bioquímica y Biología Molecular II, Facultad de Farmacia, Universidad Complutense, 28040-Madrid, Spain
*To whom correspondence should be addressed.
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Biochem J (1996) 319 (2): 627–632.
Article history
Received:
February 20 1996
Revision Received:
July 01 1996
Accepted:
July 03 1996
Citation
Teresa TERUEL, Angela M VALVERDE, Manuel BENITO, Margarita LORENZO; Insulin-like growth factor I and insulin induce adipogenic-related gene expression in fetal brown adipocyte primary cultures. Biochem J 15 October 1996; 319 (2): 627–632. doi: https://doi.org/10.1042/bj3190627
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