The modulatory effects of neuropeptide Y (NPY) on ATP-induced increases in cytosolic free-calcium concentration ([Ca2+]i) were investigated in the CHP-234 human neuroblastoma cell line. Pretreatment of cells with 100 nM NPY potentiated the increase in [Ca2+]i evoked subsequently by 20 ƁM ATP, compared with initial application of ATP in a control experiment, whereas a similar pretreatment with 1 ƁM NPY attenuated the subsequent response to ATP. Both actions of NPY were completely blocked by H-89 {N-[2-((3-(4-bromo-phenyl)-2-propenyl)-amino)-ethyl]-5 isoquinoline sulphonamide dihydrochloride}, a selective antagonist of protein kinase A. The effects of 100 nM NPY were mimicked by H-89, while forskolin and 8-Br-cAMP mimicked the effects of 1 ƁM NPY. Both basal and forskolin-stimulated cAMP levels were inhibited by 100 nM NPY and by 100 nM NPY(13-36), a selective agonist of the NPY Y2-receptor subtype. In contrast, at 1 ƁM such inhibition was not observed for either NPY or NPY(13-36). It is concluded that NPY has a biphasic modulatory effect on increases in [Ca2+]i produced by ATP, which probably involves the cAMP/protein kinase A cascade.
Skip Nav Destination
Close
Article navigation
January 1997
- Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
January 15 1997
Neuropeptide Y modulates ATP-induced increases in internal calcium via the adenylate cyclase/protein kinase A system in a human neuroblastoma cell line
Virginia SOARES LEMOS;
Virginia SOARES LEMOS
1Université Louis Pasteur de Strasbourg, Laboratoire de Pharmacologie et Physiopathologie Cellulaires-CNRS URA600, B. P. 24, F-67401 Illkirch, France
Search for other works by this author on:
Bernard BUCHER;
Bernard BUCHER
1Université Louis Pasteur de Strasbourg, Laboratoire de Pharmacologie et Physiopathologie Cellulaires-CNRS URA600, B. P. 24, F-67401 Illkirch, France
Search for other works by this author on:
Kenneth TAKEDA
Kenneth TAKEDA
*
1Université Louis Pasteur de Strasbourg, Laboratoire de Pharmacologie et Physiopathologie Cellulaires-CNRS URA600, B. P. 24, F-67401 Illkirch, France
*To whom correspondence should be addressed.
Search for other works by this author on:
Biochem J (1997) 321 (2): 439–444.
Article history
Received:
June 10 1996
Revision Received:
September 09 1996
Accepted:
September 18 1996
Citation
Virginia SOARES LEMOS, Bernard BUCHER, Kenneth TAKEDA; Neuropeptide Y modulates ATP-induced increases in internal calcium via the adenylate cyclase/protein kinase A system in a human neuroblastoma cell line. Biochem J 15 January 1997; 321 (2): 439–444. doi: https://doi.org/10.1042/bj3210439
Download citation file:
Close
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Related Articles
Autophosphorylation-dependent degradation of Pak1, triggered by the Rho-family GTPase, Chp
Biochem J (May,2007)
Role of hypothalamic neuropeptide Y and orexigenic peptides in anorexia associated with experimental colitis in the rat
Clin Sci (Lond) (January,2001)