DnaJ proteins are characterized by a ‘J‘ domain which is homologous to a region of the Escherichia coli protein DnaJ. DnaJ has been shown to interact with the chaperone protein DnaK, and a number of eukaryotic DnaJ-like proteins have been found to interact with the 70 kDa heat-shock protein/70 kDa heat-shock cognate protein (Hsp70/Hsc70), the eukaryotic homologues of DnaK. Cysteine-string proteins (Csps) are believed to function in calcium-stimulated exocytosis and in this paper we describe a specific ATP-dependent interaction between a Csp (Csp1) and Hsc70/Hsp70. We also show that Csp1 can stimulate the ATPase activity of both Hsc70 and Hsp70 several-fold. Furthermore, we demonstrate that Csp2, a Csp variant found in adrenal chromaffin cells, can enhance the ATPase activity of Hsc70 to a similar extent as Csp1, whereas Csp(137–198), a truncated protein lacking the ‘J’ domain of Csp1 is unable to stimulate the ATPase activity of Hsc70. This suggests that the functions of Csp1 and Csp2 must differ in some aspect other than interaction with Hsc70. This study is also important from a general view of DnaJ/Hsc70 interactions, as Csps lack a G/F-rich region which has been suggested to be essential for activation of the ATPase activity of DnaK by DnaJ. Thus, this work would imply that a G/F-rich region is not an essential feature of DnaJ proteins for stimulation of the ATPase activity of Hsp70 proteins.
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March 1997
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Research Article|
March 15 1997
Activation of the ATPase activity of heat-shock proteins Hsc70/Hsp70 by cysteine-string protein
Luke H. CHAMBERLAIN;
Luke H. CHAMBERLAIN
1The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, U.K.
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Robert D. BURGOYNE
Robert D. BURGOYNE
*
1The Physiological Laboratory, University of Liverpool, Crown Street, Liverpool L69 3BX, U.K.
*To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
September 26 1996
Revision Received:
October 24 1996
Accepted:
November 20 1996
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1997
1997
Biochem J (1997) 322 (3): 853–858.
Article history
Received:
September 26 1996
Revision Received:
October 24 1996
Accepted:
November 20 1996
Citation
Luke H. CHAMBERLAIN, Robert D. BURGOYNE; Activation of the ATPase activity of heat-shock proteins Hsc70/Hsp70 by cysteine-string protein. Biochem J 15 March 1997; 322 (3): 853–858. doi: https://doi.org/10.1042/bj3220853
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