The aim of the present study was to elucidate the role of mitochondria in liver impairment after ischaemia/reperfusion. It is commonly assumed that mitochondria are in part responsible for tissue damage by impaired oxidative phosphorylation as a consequence of the attack of radicals generated within the mitochondria. The principal support for this hypothesis was found by exposing isolated mitochondria to temporary hypoxia in combination with alterations of substrate supply. Rat liver mitochondria treated in this way responded with impaired ADP-stimulated respiration after reoxygenation, which decreased with time of hypoxia and reoxygenation. The decline of the activity of the NADH-cytochrome c-oxidoreductase complex found under these conditions is likely to cause the drop in active respiration. No changes in the content of respiratory chain complexes, determined by Blue Native PAGE, could be demonstrated. However, oxidative modifications of mitochondrial proteins, indicated by carbonyl formation, were found. Likewise, products of lipid peroxidation, such as lipid peroxides and malondialdehyde, were formed. Mitochondria were still able to build up a transmembrane potential and did not show drastic changes in membrane conductivity after hypoxia/reoxygenation stress. The presence of water-soluble antioxidants exhibited a beneficial effect, diminishing the decline of active respiration after 5 min of hypoxia and 10 min of reoxygenation. These observations strongly suggest that mitochondria play a pathogenic role in ischaemia/reperfusion injury, which is at least in part mediated by an oxygen-derived free-radical-linked mechanism.
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November 1997
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Research Article|
November 15 1997
Short-term impairment of energy production in isolated rat liver mitochondria by hypoxia/reoxygenation: involvement of oxidative protein modification
Lorenz SCHILD;
Lorenz SCHILD
1Otto-von-Guericke-University, Medical Faculty, Department of Pathobiochemistry, Leipziger Str. 44, 39120 Magdeburg, Germany
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Thomas REINHECKEL;
Thomas REINHECKEL
1Otto-von-Guericke-University, Medical Faculty, Department of Pathobiochemistry, Leipziger Str. 44, 39120 Magdeburg, Germany
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Ingrid WISWEDEL;
Ingrid WISWEDEL
1Otto-von-Guericke-University, Medical Faculty, Department of Pathobiochemistry, Leipziger Str. 44, 39120 Magdeburg, Germany
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Wolfgang AUGUSTIN
Wolfgang AUGUSTIN
1
1Otto-von-Guericke-University, Medical Faculty, Department of Pathobiochemistry, Leipziger Str. 44, 39120 Magdeburg, Germany
1To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
June 26 1997
Accepted:
August 07 1997
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1997
1997
Biochem J (1997) 328 (1): 205–210.
Article history
Received:
June 26 1997
Accepted:
August 07 1997
Citation
Lorenz SCHILD, Thomas REINHECKEL, Ingrid WISWEDEL, Wolfgang AUGUSTIN; Short-term impairment of energy production in isolated rat liver mitochondria by hypoxia/reoxygenation: involvement of oxidative protein modification. Biochem J 15 November 1997; 328 (1): 205–210. doi: https://doi.org/10.1042/bj3280205
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