This study investigated whether conditions known to alter the activity and phosphorylation state of the pyruvate dehydrogenase complex have specific effects on the levels of isoenzymes of pyruvate dehydrogenase kinase (PDK) in rat heart. Immunoblot analysis revealed a remarkable increase in the amount of PDK4 in the hearts of rats that had been starved or rendered diabetic with streptozotocin. Re-feeding of starved rats and insulin treatment of diabetic rats very effectively reversed the increase in PDK4 protein and restored PDK enzyme activity to levels of chow-fed control rats. Starvation and diabetes also markedly increased the abundance of PDK4 mRNA, and re-feeding and insulin treatment reduced levels of the message to that of controls. In contrast with the findings for PDK4, little or no changes in the amounts of PDK1 and PDK2 protein and the abundance of their messages occurred in response to starvation and diabetes. The observed shift in the relative abundance of PDK isoenzymes probably explains previous studies of the effects of starvation and diabetes on heart PDK activity. The results indicate that control of the amount of PDK4 is important in long-term regulation of the activity of the pyruvate dehydrogenase complex in rat heart.
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January 1998
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Research Article|
January 01 1998
Starvation and diabetes increase the amount of pyruvate dehydrogenase kinase isoenzyme 4 in rat heart
Pengfei WU;
Pengfei WU
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202-5122, U.S.A.
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Juichi SATO;
Juichi SATO
1
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202-5122, U.S.A.
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Yu ZHAO;
Yu ZHAO
2
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202-5122, U.S.A.
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Jerzy JASKIEWICZ;
Jerzy JASKIEWICZ
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202-5122, U.S.A.
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M. Kirill POPOV;
M. Kirill POPOV
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202-5122, U.S.A.
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A. Robert HARRIS
A. Robert HARRIS
3
1Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN 46202-5122, U.S.A.
3To whom all correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
May 14 1997
Revision Received:
August 26 1997
Accepted:
September 04 1997
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 329 (1): 197–201.
Article history
Received:
May 14 1997
Revision Received:
August 26 1997
Accepted:
September 04 1997
Citation
Pengfei WU, Juichi SATO, Yu ZHAO, Jerzy JASKIEWICZ, M. Kirill POPOV, A. Robert HARRIS; Starvation and diabetes increase the amount of pyruvate dehydrogenase kinase isoenzyme 4 in rat heart. Biochem J 1 January 1998; 329 (1): 197–201. doi: https://doi.org/10.1042/bj3290197
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