Sphingosine 1-phosphate (S1P) and lysophosphatidic acid (LPA) are structurally related lipid mediators that act on distinct G-protein-coupled receptors to evoke similar responses, including Ca2+ mobilization, adenylate cyclase inhibition, and mitogen-activated protein (MAP) kinase activation. However, little is still known about the respective receptors. A recently cloned putative LPA receptor (Vzg-1/Edg-2) is similar to an orphan Gi-coupled receptor termed Edg-1. Here we show that expression of Edg-1 in Sf9 and COS-7 cells results in inhibition of adenylate cyclase and activation of MAP kinase (Gi-mediated), but not Ca2+ mobilization, in response to S1P. These responses are specific in that (i) S1P action is not mimicked by LPA, and (ii) Vzg-1/Edg-2 cannot substitute for Edg-1. Thus the Edg-1 receptor is capable of mediating a subset of the cellular responses to S1P.
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March 1998
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Research Article|
March 01 1998
Sphingosine 1-phosphate signalling through the G-protein-coupled receptor Edg-1 Available to Purchase
C. M. Gerben ZONDAG;
C. M. Gerben ZONDAG
1Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
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R. Friso POSTMA;
R. Friso POSTMA
1Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
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Ingrid VAN ETTEN;
Ingrid VAN ETTEN
1Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
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Ingrid VERLAAN;
Ingrid VERLAAN
1Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
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H. Wouter MOOLENAAR
H. Wouter MOOLENAAR
1
1Division of Cellular Biochemistry, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
1To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
November 19 1997
Revision Received:
January 06 1998
Accepted:
January 09 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 330 (2): 605–609.
Article history
Received:
November 19 1997
Revision Received:
January 06 1998
Accepted:
January 09 1998
Citation
C. M. Gerben ZONDAG, R. Friso POSTMA, Ingrid VAN ETTEN, Ingrid VERLAAN, H. Wouter MOOLENAAR; Sphingosine 1-phosphate signalling through the G-protein-coupled receptor Edg-1. Biochem J 1 March 1998; 330 (2): 605–609. doi: https://doi.org/10.1042/bj3300605
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