Activated T-lymphocytes are present in early atherosclerotic lesions where they may interact with oxidized low-density lipoproteins (oxLDLs). In this study the non-specific effect of oxLDLs on the activation of T-cells in vitro was investigated. LDLs were oxidized by UV irradiation and characterized by a low level of lipid peroxidation and only slight apolipoprotein B modification. Peripheral blood lymphocytes from normal individuals were stimulated in vitro with the polyclonal activator phytohaemagglutinin in the presence of various doses of LDLs and oxLDLs. LDLs enhanced the proliferation of peripheral blood lymphocytes at doses up to 100 μg/ml but were inhibitory at 200 μg/ml, whereas low doses of oxLDLs (over 10 μg/ml) inhibited the proliferation. OxLDLs also inhibited the proliferative responses of an alloreactive CD4+ T-cell line immortalized by Herpes virus saimiri and an influenza haemagglutinin-specific CD4+ T-cell clone. Viability tests using Trypan Blue exclusion or expression of Apo2.7, an apoptosis marker, did not indicate any significant cell death at doses up to 100 μg/ml oxLDL. At this concentration, cell-cycle analysis showed an accumulation of cells at the G1/S interface in the CD4+ cell clone, without significant DNA fragmentation. The expression of the activation antigen CD25 on T-lymphocytes (on phytohaemagglutinin-activated T-cells and on CD4+ T-cell clone), requisite to the commitment of activated T-cells from G1 phase to S phase, was also inhibited by oxLDLs whereas expression of other activation antigens such as CD69 and HLA-DR was unchanged. In conclusion, these data show that mildly oxidized LDLs inhibit the proliferation and CD25 expression of activated T-lymphocytes and suggest that oxLDLs may slow down the T-cell response in atherosclerotic lesions.
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March 1998
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Research Article|
March 01 1998
Mildly oxidized low-density lipoproteins suppress the proliferation of activated CD4+ T-lymphocytes and their interleukin 2 receptor expression in vitro Available to Purchase
Sylvie CASPAR-BAUGUIL;
Sylvie CASPAR-BAUGUIL
1INSERM U 466, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse Cedex 4, France
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Majed SAADAWI;
Majed SAADAWI
1INSERM U 466, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse Cedex 4, France
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Anne NEGRE-SALVAYRE;
Anne NEGRE-SALVAYRE
1INSERM U 466, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse Cedex 4, France
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Mogens THOMSEN;
Mogens THOMSEN
1INSERM U 466, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse Cedex 4, France
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Robert SALVAYRE;
Robert SALVAYRE
1INSERM U 466, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse Cedex 4, France
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Hervé BENOIST
Hervé BENOIST
1
1INSERM U 466, Institut Louis Bugnard, CHU Rangueil, 31403 Toulouse Cedex 4, France
1To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
February 18 1997
Revision Received:
October 09 1997
Accepted:
October 10 1997
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 330 (2): 659–666.
Article history
Received:
February 18 1997
Revision Received:
October 09 1997
Accepted:
October 10 1997
Citation
Sylvie CASPAR-BAUGUIL, Majed SAADAWI, Anne NEGRE-SALVAYRE, Mogens THOMSEN, Robert SALVAYRE, Hervé BENOIST; Mildly oxidized low-density lipoproteins suppress the proliferation of activated CD4+ T-lymphocytes and their interleukin 2 receptor expression in vitro. Biochem J 1 March 1998; 330 (2): 659–666. doi: https://doi.org/10.1042/bj3300659
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