We have characterized a cDNA clone which encodes a variant (bNAT2) of the bovine noradrenaline transporter. This cDNA differs from the previously identified bovine noradrenaline transporter (bNAT1) in the sequence encoding part of the cytoplasmic-facing C-terminus and the 3ʹ-untranslated region. The bNAT1 and bNAT2 cDNA clones are encoded by a 5.8 and 3.6 kb mRNA species respectively. The bNAT1 and bNAT2 proteins, which are identical apart from their C-terminal 31 and 18 residues, were stably expressed in HEK293 cells. Cells expressing bNAT1 showed a high level of desipramine-sensitive [3H]noradrenaline uptake activity, whereas no activity was present in bNAT2 cells. The bNAT1 and bNAT2 proteins were present as major 80 and 50 kDa species respectively. Cells expressing bNAT1 showed strong immunostaining of the plasma membrane, whereas bNAT2 was present in the endoplasmic reticulum/Golgi region. Treatment of membrane samples from bNAT1 cells with peptide N-glycosidase F resulted in the formation of a predominantly 50 kDa species, but little effect was observed after similar treatment of bNAT2 cell membranes. These results indicate that bNAT2 is retained in the endoplasmic reticulum and that the glycosylation of this variant differs from that of bNAT1. The characterization of bNAT2 and its comparison with bNAT1 highlight the importance of the cytoplasmic-facing C-terminus for the intracellular trafficking of neurotransmitter transporters.
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March 1998
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Research Article|
March 01 1998
A variant of the bovine noradrenaline transporter reveals the importance of the C-terminal region for correct targeting to the membrane and functional expression Available to Purchase
D. Lucille BURTON;
D. Lucille BURTON
*Biochemistry and Molecular Biology Group, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand
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G. Andree KIPPENBERGER;
G. Andree KIPPENBERGER
*Biochemistry and Molecular Biology Group, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand
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Bettina LINGEN;
Bettina LINGEN
†Institut für Pharmakologie und Toxikologie, Universität Bonn, Reuterstrasse 2b, D-53113 Bonn, Germany
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Michael BRÜSS;
Michael BRÜSS
†Institut für Pharmakologie und Toxikologie, Universität Bonn, Reuterstrasse 2b, D-53113 Bonn, Germany
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Heinz BÖNISCH;
Heinz BÖNISCH
†Institut für Pharmakologie und Toxikologie, Universität Bonn, Reuterstrasse 2b, D-53113 Bonn, Germany
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L. David CHRISTIE
L. David CHRISTIE
1
*Biochemistry and Molecular Biology Group, School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand
1To whom correspondence should be addressed.
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Publisher: Portland Press Ltd
Received:
July 22 1997
Revision Received:
September 22 1997
Accepted:
October 27 1997
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 330 (2): 909–914.
Article history
Received:
July 22 1997
Revision Received:
September 22 1997
Accepted:
October 27 1997
Citation
D. Lucille BURTON, G. Andree KIPPENBERGER, Bettina LINGEN, Michael BRÜSS, Heinz BÖNISCH, L. David CHRISTIE; A variant of the bovine noradrenaline transporter reveals the importance of the C-terminal region for correct targeting to the membrane and functional expression. Biochem J 1 March 1998; 330 (2): 909–914. doi: https://doi.org/10.1042/bj3300909
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