We have previously shown that dietary sucrose stimulates the lactase/phlorizin hydrolase (LPH) mRNA accumulation along with a rise in lactase activity in rat jejunum [Goda, Yasutake, Suzuki, Takase and Koldovský (1995) Am. J. Physiol. 268, G1066–G1073]. To elucidate the mechanisms whereby dietary carbohydrates enhance the LPH mRNA expression, 7-week-old rats that had been fed a low-carbohydrate diet (5.5% of energy as starch) were given diets containing various monosaccharides or sucrose for 12 h. Among carbohydrates examined, fructose, sucrose, galactose and glycerol elicited an increase in LPH mRNA accumulation along with a rise in lactase activity in the jejunum. By contrast, glucose and α-methylglucoside were unable to elicit a significant increase in LPH mRNA levels. To explore a transcriptional mechanism for the carbohydrate-induced increases in LPH mRNA levels, we employed two techniques currently available to estimate transcriptional rate, i.e. RNA protection assays of pre-mRNA using an intron probe, and nuclear run-on assays. Both assays revealed that fructose elicited an increase in transcription of the LPH gene, and that the transcription of LPH was influenced only slightly, if at all, by glucose intake. These results suggest that certain monosaccharides such as fructose or their metabolite(s) are capable of enhancing LPH mRNA levels in the small intestine, and that transcriptional control might play a major role in the carbohydrate-induced increase of LPH mRNA expression.
Skip Nav Destination
Follow us on Twitter @Biochem_Journal
Article navigation
April 1998
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
April 01 1998
Dietary carbohydrates enhance lactase/phlorizin hydrolase gene expression at a transcription level in rat jejunum
Takemi TANAKA;
Takemi TANAKA
1Department of Nutrition, School of Food and Nutritional Sciences, The University of Shizuoka, 52-1 Yada, Shizuoka 422, Japan
Search for other works by this author on:
Kazuhiro KISHI;
Kazuhiro KISHI
1Department of Nutrition, School of Food and Nutritional Sciences, The University of Shizuoka, 52-1 Yada, Shizuoka 422, Japan
Search for other works by this author on:
Makiko IGAWA;
Makiko IGAWA
1Department of Nutrition, School of Food and Nutritional Sciences, The University of Shizuoka, 52-1 Yada, Shizuoka 422, Japan
Search for other works by this author on:
Sachiko TAKASE;
Sachiko TAKASE
1Department of Nutrition, School of Food and Nutritional Sciences, The University of Shizuoka, 52-1 Yada, Shizuoka 422, Japan
Search for other works by this author on:
Toshinao GODA
Toshinao GODA
1
1Department of Nutrition, School of Food and Nutritional Sciences, The University of Shizuoka, 52-1 Yada, Shizuoka 422, Japan
1To whom correspondence should be addressed.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
November 03 1997
Accepted:
December 16 1997
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 331 (1): 225–230.
Article history
Received:
November 03 1997
Accepted:
December 16 1997
Citation
Takemi TANAKA, Kazuhiro KISHI, Makiko IGAWA, Sachiko TAKASE, Toshinao GODA; Dietary carbohydrates enhance lactase/phlorizin hydrolase gene expression at a transcription level in rat jejunum. Biochem J 1 April 1998; 331 (1): 225–230. doi: https://doi.org/10.1042/bj3310225
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Follow us on Twitter @Biochem_Journal
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() View past webinars > |