We have cloned and sequenced genomic DNA from a human library extending 1300 bp upstream the 5´-untranslated sequence of the cDNA coding for the sodium/iodide symporter. In transient transfection assays this sequence exhibited promoter activity, which could be confined to nucleotides -443 to -395 relative to the ATG start codon. This minimal promoter, including a putative GC- and TATA- box, was preferentially activated in the rat thyroid cell line FRTL-5, but was also active in non-thyroidal cells, such as COS-7 and Chinese-hamster ovary, albeit to a markedly lower extent.
Research Article| April 15 1998
Cloning of a functional promoter of the human sodium/iodide-symporter gene
Thomas L. SCHMITT;
Celia R. ESPINOZA;
Ulrich LOOS 1
1Abteilung Innere Medizin I, University of Ulm, Universitätsklinikum Ulm, Robert-Koch-Strasse 8, 89081 Ulm, Federal Republic of Germany
1To whom correspondence should be addressed (e-mail Ulrich.Loos@medizin.uni-ulm.de).
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Michael BEHR, Thomas L. SCHMITT, Celia R. ESPINOZA, Ulrich LOOS; Cloning of a functional promoter of the human sodium/iodide-symporter gene. Biochem J 15 April 1998; 331 (2): 359–363. doi: https://doi.org/10.1042/bj3310359
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