The N-terminal amino acid sequences of proteolytic fragments of neuropathy target esterase (NTE), covalently labelled on its active-site serine by a biotinylated organophosphorus ester, were determined and used to deduce the location of this serine residue and to initiate cloning of its cDNA. A putative NTE clone, isolated from a human foetal brain cDNA library, encoded a 1327 residue polypeptide with no homology to any known serine esterases or proteases. The active-site serine of NTE (Ser-966) lay in the centre of a predicted hydrophobic helix within a 200-amino-acid C-terminal domain with marked similarity to conceptual proteins in bacteria, yeast and nematodes; these proteins may comprise a novel family of potential serine hydrolases. The Swiss Cheese protein which, when mutated, leads to widespread cell death in Drosophilabrain [Kretzschmar, Hasan, Sharma, Heisenberg and Benzer (1997) J. Neurosci. 17, 7425–7432], was strikingly homologous to NTE, suggesting that genetically altered NTE may be involved in human neurodegenerative disease.
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May 1998
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Research Article|
May 15 1998
Neuropathy target esterase and a homologous Drosophila neurodegeneration-associated mutant protein contain a novel domain conserved from bacteria to man Available to Purchase
Michael J. LUSH;
Michael J. LUSH
*MRC Toxicology Unit, University of Leicester, Leicester LE1 9HN, U.K.
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Yong LI;
Yong LI
*MRC Toxicology Unit, University of Leicester, Leicester LE1 9HN, U.K.
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David J. READ;
David J. READ
*MRC Toxicology Unit, University of Leicester, Leicester LE1 9HN, U.K.
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Anthony C. WILLIS;
Anthony C. WILLIS
†MRC Immunochemistry Unit, University of Oxford, Oxford OX1 3QU, U.K.
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Paul GLYNN
Paul GLYNN
1
*MRC Toxicology Unit, University of Leicester, Leicester LE1 9HN, U.K.
1To whom correspondence should be addressed (e-mail [email protected]).
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Publisher: Portland Press Ltd
Received:
March 02 1998
Accepted:
March 18 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 332 (1): 1–4.
Article history
Received:
March 02 1998
Accepted:
March 18 1998
Citation
Michael J. LUSH, Yong LI, David J. READ, Anthony C. WILLIS, Paul GLYNN; Neuropathy target esterase and a homologous Drosophila neurodegeneration-associated mutant protein contain a novel domain conserved from bacteria to man. Biochem J 15 May 1998; 332 (1): 1–4. doi: https://doi.org/10.1042/bj3320001
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