The metallo-β-lactamase produced by Chryseobacterium(formerly Flavobacterium) meningosepticum,which is the flavobacterial species of greatest clinical relevance, was purified and characterized. The enzyme, named BlaB, contains a polypeptide with an apparent Mr of 26000, and has a pI of 8.5. It hydrolyses penicillins, cephalosporins (including cefoxitin), carbapenems and 6-β-iodopenicillanate, a mechanism-based inactivator of active-site serine β-lactamases. The enzyme was inhibited by EDTA, 1-10 phenanthroline and pyridine-2,6-dicarboxylic acid, with different inactivation parameters for each chelating agent. The C. meningosepticum blaBgene was cloned and sequenced. According to the G+C content and codon usage, the blaBgene appeared to be endogenous to the species. The BlaB enzyme showed significant sequence similarity to other class B β-lactamases, being overall more similar to members of subclass B1, which includes the metallo-enzymes of Bacillus cereus(Bc-II) and Bacteroides fragilis(CcrA) and the IMP-1 enzyme found in various microbial species, and more distantly related to the metallo-β-lactamases of Aeromonasspp. (CphA, CphA2 and ImiS) and of Stenotrophomonas maltophilia(L1).
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May 1998
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Research Article|
May 15 1998
Characterization and sequence of the Chryseobacterium (Flavobacterium) meningosepticum carbapenemase: a new molecular class B β-lactamase showing a broad substrate profile Available to Purchase
Gian Maria ROSSOLINI;
Gian Maria ROSSOLINI
1
*Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 – Siena, Italy
1To whom correspondence should be addressed (e-mail [email protected]).
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Nicola FRANCESCHINI;
Nicola FRANCESCHINI
†Dipartimento di Scienze e Tecnologie Biomediche e Biometria, Università dell'Aquila, 67100 – Loc. Coppito, L'Aquila, Italy
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Maria Letizia RICCIO;
Maria Letizia RICCIO
*Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Università di Siena, 53100 – Siena, Italy
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Paola Sandra MERCURI;
Paola Sandra MERCURI
†Dipartimento di Scienze e Tecnologie Biomediche e Biometria, Università dell'Aquila, 67100 – Loc. Coppito, L'Aquila, Italy
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Mariagrazia PERILLI;
Mariagrazia PERILLI
†Dipartimento di Scienze e Tecnologie Biomediche e Biometria, Università dell'Aquila, 67100 – Loc. Coppito, L'Aquila, Italy
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Moreno GALLENI;
Moreno GALLENI
‡Centre d'Ingénierie des Protéines, Université de Liège, Sart Tilman, B-4000 – Liège, Belgium
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Jean-Marie FRERE;
Jean-Marie FRERE
‡Centre d'Ingénierie des Protéines, Université de Liège, Sart Tilman, B-4000 – Liège, Belgium
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Gianfranco AMICOSANTE
Gianfranco AMICOSANTE
†Dipartimento di Scienze e Tecnologie Biomediche e Biometria, Università dell'Aquila, 67100 – Loc. Coppito, L'Aquila, Italy
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Publisher: Portland Press Ltd
Received:
January 05 1998
Accepted:
February 13 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 332 (1): 145–152.
Article history
Received:
January 05 1998
Accepted:
February 13 1998
Citation
Gian Maria ROSSOLINI, Nicola FRANCESCHINI, Maria Letizia RICCIO, Paola Sandra MERCURI, Mariagrazia PERILLI, Moreno GALLENI, Jean-Marie FRERE, Gianfranco AMICOSANTE; Characterization and sequence of the Chryseobacterium (Flavobacterium) meningosepticum carbapenemase: a new molecular class B β-lactamase showing a broad substrate profile. Biochem J 15 May 1998; 332 (1): 145–152. doi: https://doi.org/10.1042/bj3320145
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