In a previous study, evidence was provided for the presence of a novel plasma-membrane-associated neutral-pH-optimum α-l-fucosidase in rat sperm. In the present study, rat sperm α-l-fucosidase was characterized during epididymal maturation. The pH 7 activity optimum of α-l-fucosidase and its subunit composition (one or two closely spaced immunoreactive protein bands of about 53±2 kDa) did not appear to change during transit through the epididymis. Isoelectric focusing of α-l-fucosidase indicated the presence of a major isoform (B) with a pI near 7 in sperm from testis, caput, corpus and the proximal half of the cauda. α-l-Fucosidase from sperm from the distal half of the cauda, which contained a significant enrichment of sperm and α-l-fucosidase activity, contained isoform B and an additional minor isoform (A) with a pI near 5.2. Isoform B and small amounts of isoform A were present in sperm from the vas deferens. The two fucosidase isoforms present in sperm from the distal cauda were separated by isoelectric focusing and comparatively characterized. They had similar pH–activity curves (with optima near pH 7) and comparable apparent KM values (0.4±0.04 mM) for 4-methylumbelliferyl α-l-fucopyranoside. Preincubation of the isoforms at different temperatures indicated that isoform A is considerably more thermostable than isoform B. Immunoprecipitation studies using polyclonal antibodies against human liver α-l-fucosidase indicated that approx. 90% of the enzymic activity for both isoforms was immunoprecipitable under conditions that immunoprecipitated essentially all the human liver enzyme. Neuraminidase treatment of sperm α-l-fucosidase from distal cauda (when compared with the appropriate heat-treated control) led to disappearance of isoform A and a concomitant increase in isoform B. The overall results suggest that isoform A is derived by sialylation of isoform B near the end of epididymal maturation.
Alteration of the isoform composition of plasma-membrane-associated rat sperm α-l-fucosidase during late epididymal maturation: comparative characterization of the acidic and neutral isoforms
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Irene ABASCAL, Sheri R. SKALABAN, Karen M. GRIMM, Manuel AVILÉS, José Angel MARTÍNEZ-MENÁRGUEZ, Maria Teresa CASTELLS, José BALLESTA, Jack A. ALHADEFF; Alteration of the isoform composition of plasma-membrane-associated rat sperm α-l-fucosidase during late epididymal maturation: comparative characterization of the acidic and neutral isoforms. Biochem J 1 July 1998; 333 (1): 201–207. doi: https://doi.org/10.1042/bj3330201
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