Signalling through the leukotriene B4 receptor involves both αi and α16, but not αq or α11 G-protein subunits

COS-7 cells transfected with the leukotriene (LT) B₄ receptor (BLTR) cDNA were unable to produce LTB₄-induced inositol phosphates (IPs) in spite of the presence of endogenous Gα_i, Gα_q and Gα₁₁ proteins. Co-transfection of BLTR with Gα₁₆, however, resulted in high levels of IP production, which were 17-, 10- and 6-fold higher than with co-transfected Gα₁₁, Gα_q and Gα₁₄, respectively. Co-transfection of BLTR with phospholipase C (PLC) β₂, on the other hand, resulted in efficient IP production and co-transfection of BLTR with both Gα₁₆ and PLCβ₂ resulted in a greater than additive response.