We report the isolation and the functional characterization of α and β chains from pig (Sus scropha domesticus) haemoglobin, as well as of the pig–human hybrid haemoglobins, α2hβ2p and α2pβ2h (i.e. Circe's haemoglobins), obtained by mixing the purified α and β pig chains respectively with the corresponding partner human chains. Their functional properties have been compared with those of both parental haemoglobins in order to obtain information on the role of the different subunits and of their inter-relationships, both at the structural and functional levels. The results indicate that the functional properties of both hybrids are closer to those of the parental haemoglobin that provides the β chains, confirming the major role of the β chains in determining the oxygen affinity and the modulation mechanisms of the tetrameric molecule. This is supported by the thermodynamic properties, since the very low ΔH of oxygen binding that characterizes pig haemoglobin and the α2hβ2p hybrid haemoglobin may be taken as the reflection of specific structural properties of pig β chain.
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October 1998
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Research Article|
October 15 1998
Circe's haemoglobins, pig–human hybrids: functional characterization and structural considerations Available to Purchase
Maria T. SANNA;
Maria T. SANNA
*Department of Biochemistry and Human Physiology, University of Cagliari, Cittadella Universitaria, S. Prov.le Monserrato-Sestu Km 0.7, 09042 Monserrato (CA), Italy
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Bruno GIARDINA;
Bruno GIARDINA
†CNR Center for Receptor Chemistry and Institute of Chemistry and Clinical Chemistry, Catholic University, Largo Francesco Vito 1, 00168 Roma, Italy
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Mariagiuseppina PELLEGRINI;
Mariagiuseppina PELLEGRINI
*Department of Biochemistry and Human Physiology, University of Cagliari, Cittadella Universitaria, S. Prov.le Monserrato-Sestu Km 0.7, 09042 Monserrato (CA), Italy
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Alessandra OLIANAS;
Alessandra OLIANAS
*Department of Biochemistry and Human Physiology, University of Cagliari, Cittadella Universitaria, S. Prov.le Monserrato-Sestu Km 0.7, 09042 Monserrato (CA), Italy
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Irene MESSANA;
Irene MESSANA
†CNR Center for Receptor Chemistry and Institute of Chemistry and Clinical Chemistry, Catholic University, Largo Francesco Vito 1, 00168 Roma, Italy
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Massimo CASTAGNOLA;
Massimo CASTAGNOLA
*Department of Biochemistry and Human Physiology, University of Cagliari, Cittadella Universitaria, S. Prov.le Monserrato-Sestu Km 0.7, 09042 Monserrato (CA), Italy
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Marcella CORDA
Marcella CORDA
1
*Department of Biochemistry and Human Physiology, University of Cagliari, Cittadella Universitaria, S. Prov.le Monserrato-Sestu Km 0.7, 09042 Monserrato (CA), Italy
1To whom correspondence should be addressed (e-mail [email protected]).
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Publisher: Portland Press Ltd
Received:
January 12 1998
Revision Received:
July 13 1998
Accepted:
July 30 1998
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1998
1998
Biochem J (1998) 335 (2): 211–216.
Article history
Received:
January 12 1998
Revision Received:
July 13 1998
Accepted:
July 30 1998
Citation
Maria T. SANNA, Bruno GIARDINA, Mariagiuseppina PELLEGRINI, Alessandra OLIANAS, Irene MESSANA, Massimo CASTAGNOLA, Marcella CORDA; Circe's haemoglobins, pig–human hybrids: functional characterization and structural considerations. Biochem J 15 October 1998; 335 (2): 211–216. doi: https://doi.org/10.1042/bj3350211
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