The role of plasmalogen phospholipids for copper-induced lipid oxidation was evaluated. Using 1H-NMR we observed that the copper (CuSO4)-promoted oxidative degradation of polyunsaturated fatty acids in micellar solution was dose-dependently attenuated by the plasmalogen lysoplasmenylethanolamine from bovine brain (lysoBP-PtdEtn). This was due to a direct interaction of copper ions with the plasmalogen-specific enol ether double bond. The enol ether methine 1H signal decreased on the addition of copper, saturation being reached at a molar ratio of lysoBP-PtdEtn to copper of 1:1. The original 1H signal was recovered almost completely after the addition of EDTA. Enrichment of micelles and low-density lipoproteins (LDLs) with plasmalogen phospholipids led to a decrease in the Cu(II) concentration in the aqueous media. After loading of LDLs in vitro with BP-PtdEtn, the LDL-dependent formation of Cu(I) was decreased, in particular in particles experimentally supplemented with α-tocopherol. The suppression of copper-promoted lipid oxidation that was observed in the presence of plasmalogen phospholipids plus α-tocopherol was greater than the sum of the protective effects elicited by the two substances alone. In conclusion, the formation of a complex between copper ions and the plasmalogens accounts partly for their inhibition of copper-induced lipid oxidation.
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June 1999
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Research Article|
May 25 1999
Contribution of copper binding to the inhibition of lipid oxidation by plasmalogen phospholipids
Daniela HAHNEL;
Daniela HAHNEL
*Physiologisches Institut der Universität München, Pettenkoferstrasse 12, D-80336 München, Germany
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Thomas HUBER;
Thomas HUBER
†Institut für Physikalische Biochemie der Universität München, Schillerstrasse 44, D-80336 München, Germany
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Volker KURZE;
Volker KURZE
†Institut für Physikalische Biochemie der Universität München, Schillerstrasse 44, D-80336 München, Germany
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Klaus BEYER;
Klaus BEYER
†Institut für Physikalische Biochemie der Universität München, Schillerstrasse 44, D-80336 München, Germany
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Bernd ENGELMANN
Bernd ENGELMANN
1
*Physiologisches Institut der Universität München, Pettenkoferstrasse 12, D-80336 München, Germany
1To whom correspondence should be addressed (e-mail [email protected]).
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Publisher: Portland Press Ltd
Received:
December 04 1998
Revision Received:
February 26 1999
Accepted:
March 22 1999
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1999
1999
Biochem J (1999) 340 (2): 377–383.
Article history
Received:
December 04 1998
Revision Received:
February 26 1999
Accepted:
March 22 1999
Citation
Daniela HAHNEL, Thomas HUBER, Volker KURZE, Klaus BEYER, Bernd ENGELMANN; Contribution of copper binding to the inhibition of lipid oxidation by plasmalogen phospholipids. Biochem J 1 June 1999; 340 (2): 377–383. doi: https://doi.org/10.1042/bj3400377
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