The intralobular distribution of metabolism was examined in the livers from rats with severe diabetic ketoacidosis (DKA), perfused at pH 6.8, and compared with that in livers from normal starved animals perfused at either pH 7.4 or 6.8. With lactate and palmitate as substrates, the perivenous uptake of periportally synthesized glucose seen in normal livers at pH 7.4 was abolished during DKA; indeed, gluconeogenesis was most active in the perivenous region. Whereas in normal livers perfused at pH 7.4 the periportal region showed a markedly elevated intracellular pH (pHi) compared with the perivenous zone, this distribution of pHi was reversed in DKA, with an intermediate distribution in normal livers perfused at pH 6.8. 3-Hydroxybutyrate was generated throughout the lobule. Some acetoacetate generated periportally was converted to 3-hydroxybutyrate more perivenously. A steep gradient of oxygen uptake along the radius of the lobule was apparent in all three groups; oxygen uptake was greatly decreased perivenously despite adequate oxygen supply. These findings are consistent with our previous observations of the lobular co-location of high pHi and gluconeogenesis, and might offer an explanation of how high gluconeogenic rates can continue in spite of severe systemic acidosis in DKA. The findings provide direct evidence for a marked redistribution of intralobular metabolism in DKA.
Skip Nav Destination
Follow us on Twitter @Biochem_Journal
Article navigation
October 1999
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
Research Article|
September 24 1999
Zonation of gluconeogenesis, ketogenesis and intracellular pH in livers from normal and diabetic ketoacidotic rats: evidence for intralobular redistribution of metabolic events in ketoacidosis
Shamus P. BURNS;
Shamus P. BURNS
1
*Medical Unit (Whitechapel), St Bartholomew's and the Royal London Hospital School of Medicine and Dentistry, London E1 1BB, U.K.
1To whom correspondence should be addressed (e-mail [email protected]).
Search for other works by this author on:
Robert D. COHEN;
Robert D. COHEN
*Medical Unit (Whitechapel), St Bartholomew's and the Royal London Hospital School of Medicine and Dentistry, London E1 1BB, U.K.
Search for other works by this author on:
Richard A. ILES;
Richard A. ILES
*Medical Unit (Whitechapel), St Bartholomew's and the Royal London Hospital School of Medicine and Dentistry, London E1 1BB, U.K.
Search for other works by this author on:
Rosemary A. BAILEY;
Rosemary A. BAILEY
†School of Mathematical Sciences, Queen Mary and Westfield College, London E1 4NS, U.K.
Search for other works by this author on:
Mina DESAI;
Mina DESAI
‡Department of Clinical Biochemistry, University of Cambridge Clinical School, Addenbrooke's Hospital, Cambridge CB2 2QR, U.K.
Search for other works by this author on:
Jocelyn P. GERMAIN;
Jocelyn P. GERMAIN
§Department of Oral Pathology, St Bartholomew's and the Royal London Hospital School of Medicine and Dentistry, London E1 1BB, U.K.
Search for other works by this author on:
Thomas C. H. GOING
Thomas C. H. GOING
*Medical Unit (Whitechapel), St Bartholomew's and the Royal London Hospital School of Medicine and Dentistry, London E1 1BB, U.K.
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
April 12 1999
Revision Received:
July 01 1999
Accepted:
July 27 1999
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 1999
1999
Biochem J (1999) 343 (1): 273–280.
Article history
Received:
April 12 1999
Revision Received:
July 01 1999
Accepted:
July 27 1999
Citation
Shamus P. BURNS, Robert D. COHEN, Richard A. ILES, Rosemary A. BAILEY, Mina DESAI, Jocelyn P. GERMAIN, Thomas C. H. GOING; Zonation of gluconeogenesis, ketogenesis and intracellular pH in livers from normal and diabetic ketoacidotic rats: evidence for intralobular redistribution of metabolic events in ketoacidosis. Biochem J 1 October 1999; 343 (1): 273–280. doi: https://doi.org/10.1042/bj3430273
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Follow us on Twitter @Biochem_Journal
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() View past webinars > |