The carboxyl ester lipase (CEL) gene is highly expressed in exocrine pancreas and expression of the human CEL gene is mediated by a strong tissue-specific enhancer, which is absolutely necessary for high-level expression. The mouse promoter, on the other hand, does not contain a corresponding enhancer element, but instead is totally dependent on another pancreas-specific element. This element is identified as a pancreatic transcription factor 1 (PTF 1)-binding site. The human CEL promoter also contains a putative PTF 1 element located at a position corresponding to the essential PTF 1 site in the mouse promoter. However, nucleotide changes in the human promoter 5´ flanking this PTF 1 site have created an overlapping CCAAT/enhancer-binding protein (C/EBP)-like binding motif, interfering with the binding of PTF 1. Hence, our findings provide an example of genetic divergence between species not accompanied by difference in function.
The tissue-specific regulation of the carboxyl ester lipase gene in exocrine pancreas differs significantly between mouse and human
- Views Icon Views
- Share Icon Share
- Cite Icon Cite
Marie KANNIUS-JANSON, Ulf LIDBERG, Gunnar BJURSELL, Jeanette NILSSON; The tissue-specific regulation of the carboxyl ester lipase gene in exocrine pancreas differs significantly between mouse and human. Biochem J 15 October 2000; 351 (2): 367–376. doi: https://doi.org/10.1042/bj3510367
Download citation file: