The carboxyl ester lipase (CEL) gene is highly expressed in exocrine pancreas and expression of the human CEL gene is mediated by a strong tissue-specific enhancer, which is absolutely necessary for high-level expression. The mouse promoter, on the other hand, does not contain a corresponding enhancer element, but instead is totally dependent on another pancreas-specific element. This element is identified as a pancreatic transcription factor 1 (PTF 1)-binding site. The human CEL promoter also contains a putative PTF 1 element located at a position corresponding to the essential PTF 1 site in the mouse promoter. However, nucleotide changes in the human promoter 5´ flanking this PTF 1 site have created an overlapping CCAAT/enhancer-binding protein (C/EBP)-like binding motif, interfering with the binding of PTF 1. Hence, our findings provide an example of genetic divergence between species not accompanied by difference in function.
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October 2000
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Research Article|
October 10 2000
The tissue-specific regulation of the carboxyl ester lipase gene in exocrine pancreas differs significantly between mouse and human
Marie KANNIUS-JANSON;
Marie KANNIUS-JANSON
1Department of CMB/Molecular Biology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden
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Ulf LIDBERG;
Ulf LIDBERG
1Department of CMB/Molecular Biology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden
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Gunnar BJURSELL;
Gunnar BJURSELL
1Department of CMB/Molecular Biology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden
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Jeanette NILSSON
Jeanette NILSSON
1
1Department of CMB/Molecular Biology, Göteborg University, Box 462, S-405 30 Göteborg, Sweden
1To whom correspondence should be addressed (e-mail Jeanette.Nilsson@molbio.gu.se).
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Publisher: Portland Press Ltd
Received:
April 19 2000
Revision Received:
June 29 2000
Accepted:
August 04 2000
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2000
2000
Biochem J (2000) 351 (2): 367–376.
Article history
Received:
April 19 2000
Revision Received:
June 29 2000
Accepted:
August 04 2000
Citation
Marie KANNIUS-JANSON, Ulf LIDBERG, Gunnar BJURSELL, Jeanette NILSSON; The tissue-specific regulation of the carboxyl ester lipase gene in exocrine pancreas differs significantly between mouse and human. Biochem J 15 October 2000; 351 (2): 367–376. doi: https://doi.org/10.1042/bj3510367
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