Non-enzymic damage to nuclear proteins has potentially severe consequences for the maintenance of genomic integrity. Introduction of carbonyl groups into histones in vivo and in vitro was assessed by Western blot immunoassay and reductive incorporation of tritium from radiolabelled NaBH4 (sodium borohydride). Histone H1 extracted from bovine thymus, liver and spleen was found to contain significantly elevated amounts of protein-bound carbonyl groups as compared with core histones. The carbonyl content of nuclear proteins of rat pheochromocytoma cells (PC12 cells) was not greatly increased following oxidative stress induced by H2O2, but was significantly increased following alkylating stress induced by N-methyl-N´-nitro-N-nitrosoguanidine or by combined oxidative and alkylating stress. Free ADP-ribose, a reducing sugar generated in the nucleus in proportion to DNA strand breaks, was shown to be a potent histone H1 carbonylating agent in isolated PC12 cell nuclei. Studies of the mechanism of histone H1 modification by ADP-ribose indicate that carbonylation involves formation of a stable acyclic ketoamine. Our results demonstrate preferential histone H1 carbonylation in vivo, with potentially important consequences for chromatin structure and function.
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November 2000
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Research Article|
October 24 2000
Histone carbonylation in vivo and in vitro Available to Purchase
Georg T. WONDRAK;
Georg T. WONDRAK
*College of Pharmacy, University of Kentucky, Lexington, KY 40506-0286, U.S.A.
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Daniel CERVANTES-LAUREAN;
Daniel CERVANTES-LAUREAN
†Department of Clinical Sciences, University of Kentucky, Lexington, KY 40506-0286, U.S.A.
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Elaine L. JACOBSON;
Elaine L. JACOBSON
†Department of Clinical Sciences, University of Kentucky, Lexington, KY 40506-0286, U.S.A.
‡Lucille P. Markey Cancer Center, University of Kentucky, Lexington, KY 40506-0286, U.S.A.
§Advanced Science and Technology Commercialization Center, University of Kentucky, Lexington, KY 40506-0286, U.S.A.
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Myron K. JACOBSON
Myron K. JACOBSON
1
*College of Pharmacy, University of Kentucky, Lexington, KY 40506-0286, U.S.A.
‡Lucille P. Markey Cancer Center, University of Kentucky, Lexington, KY 40506-0286, U.S.A.
§Advanced Science and Technology Commercialization Center, University of Kentucky, Lexington, KY 40506-0286, U.S.A.
1To whom correspondence should be addressed (e-mail [email protected]).
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Publisher: Portland Press Ltd
Received:
May 08 2000
Revision Received:
June 26 2000
Accepted:
July 24 2000
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London © 2000
2000
Biochem J (2000) 351 (3): 769–777.
Article history
Received:
May 08 2000
Revision Received:
June 26 2000
Accepted:
July 24 2000
Citation
Georg T. WONDRAK, Daniel CERVANTES-LAUREAN, Elaine L. JACOBSON, Myron K. JACOBSON; Histone carbonylation in vivo and in vitro. Biochem J 1 November 2000; 351 (3): 769–777. doi: https://doi.org/10.1042/bj3510769
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