We previously reported [Xie, Li, Méchin and van de Werve (1999) Biochem. J. 343, 393–396] that dietary phosphate deprivation for 2 days up-regulated both the catalytic subunit and the putative glucose-6-phosphate translocase of the rat liver microsomal glucose-6-phosphatase system, suggesting that increased hepatic glucose production might be responsible for the frequent clinical association of hypophosphataemia and glucose intolerance. We now show that liver cAMP was increased in rats fed with a diet deficient in Pi compared with rats fed with a control diet. Accordingly, in the Pi-deficient group pyruvate kinase was inactivated, the concentration of phosphoenolpyruvate was increased and fructose 2,6-bisphosphate concentration was decreased. Phosphoenolpyruvate carboxykinase activity was marginally increased and glucokinase activity was unchanged by Pi deprivation. The liver glycogen concentration decreased in the Pi-deficient group. In the fed state, plasma glucose concentration was increased and plasma Pi and insulin concentrations were substantially decreased in the Pi-deficient group. All of these changes, except decreased plasma Pi, were cancelled in the overnight fasted Pi-deficient group. In the fasted Pi-deficient group, immediately after a glucose bolus, the plasma glucose level was elevated and the inhibition of endogenous glucose production was decreased. However, this mild glucose intolerance was not sufficient to affect the rate of fall of the glucose level after the glucose bolus. Taken together, these changes are compatible with a stimulation of liver gluconeogenesis and glycogenolysis by the Pi-deficient diet and further indicate that the liver might contribute to impaired glucose homeostasis in Pi-deficient states.
Dietary Pi deprivation in rats affects liver cAMP, glycogen, key steps of gluconeogenesis and glucose production
- Views Icon Views
- Share Icon Share
Wensheng XIE, T. Luong TRAN, Diane T. FINEGOOD, Gérald VAN DE WERVE; Dietary Pi deprivation in rats affects liver cAMP, glycogen, key steps of gluconeogenesis and glucose production. Biochem J 15 November 2000; 352 (1): 227–232. doi: https://doi.org/10.1042/bj3520227
Download citation file: