We have studied the degree to which fluorescent Ca2+ indicator dyes, and green fluorescent protein and its variants, can be used together. We find that the most commonly used fluorescent protein, enhanced green fluorescent protein (EGFP), seriously contaminates fura 2 signals. We suggest two alternative combinations for which there is no detectable contamination of the Ca2+ indicator signal by the fluorescent protein. Blue fluorescent protein can be used with the Ca2+ indicator Fura Red; EGFP can be used with the Ca2+ indicator X-Rhod 1. The use of these combinations will permit the accurate measurement of Ca2+ signals in cells transfected with fluorescent proteins.
Use of fluorescent Ca2+ dyes with green fluorescent protein and its variants: problems and solutions
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Stephen BOLSOVER, Ozbek IBRAHIM, Niamh O'LUANAIGH, Helen WILLIAMS, Shamshad COCKCROFT; Use of fluorescent Ca2+ dyes with green fluorescent protein and its variants: problems and solutions. Biochem J 1 June 2001; 356 (2): 345–352. doi: https://doi.org/10.1042/bj3560345
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