Cytokines are small proteins that have an essential role in the immune and inflammatory responses. The repertoire of cytokines is becoming diverse and expanding. Here we report the identification and characterization of a novel cytokine designated as chemokine-like factor 1 (CKLF1). The full-length cDNA of CKLF1 is 530bp long and a single open reading frame encoding 99 amino acid residues. CKLF1 bears no significant similarity to any other known cytokine in its amino acid sequence. Expression of CKLF1 can be partly inhibited by interleukin 10 in PHA-stimulated U937 cells. Recombinant CKLF1 is a potent chemoattractant for neutrophils, monocytes and lymphocytes; moreover, it can stimulate the proliferation of murine skeletal muscle cells. These results suggest that CKLF1 might have important roles in inflammation and in the regeneration of skeletal muscle.
Molecular cloning and characterization of chemokine-like factor 1 (CKLF1), a novel human cytokine with unique structure and potential chemotactic activity
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Wenling HAN, Yaxin LOU, Junmin TANG, Yingmei ZHANG, Yingyu CHEN, Ying LI, Weifeng GU, Jiaqiang HUANG, Liming GUI, Yan TANG, Feng LI, Quansheng SONG, Chunhui DI, Lu WANG, Qun SHI, Ronghua SUN, Donglan XIA, Min RUI, Jian TANG, Dalong MA; Molecular cloning and characterization of chemokine-like factor 1 (CKLF1), a novel human cytokine with unique structure and potential chemotactic activity. Biochem J 1 July 2001; 357 (1): 127–135. doi: https://doi.org/10.1042/bj3570127
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