An Australian common brown snake, Pseudonaja textilis, is known to contain highly lethal neurotoxins. Among them, a long-chain α-neurotoxin, pseudonajatoxin b, has been identified. In this report, while presenting evidence for the presence of at least four such long-chain α-neurotoxins in the venom of P. textilis, we describe the characteristics of both the mRNA and the gene responsible for the synthesis of these neurotoxins. A precursor toxin synthesized from the gene has been identified as being capable of producing the isoforms possibly by post-translational modifications at its C-terminal end. Recombinant toxins corresponding to the precursor and its product have been found to possess similar binding affinities for muscular acetylcholine receptors (IC50 = 3×10−8 M) and a lethality, LD50, of 0.15μg/g in mice.
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Research Article| September 10 2001
Cloning and characterization of the pseudonajatoxin b precursor
Kandiah JEYASEELAN 1
∗Department of Biochemistry, Faculty of Medicine, Faculty of Science, National University of Singapore, 10 Medical Drive, Singapore 119260
1To whom correspondence should be addressed (e-mail firstname.lastname@example.org).
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NanLing GONG, Arunmozhiarasi ARMUGAM, Peter MIRTSCHIN, Kandiah JEYASEELAN; Cloning and characterization of the pseudonajatoxin b precursor. Biochem J 15 September 2001; 358 (3): 647–656. doi: https://doi.org/10.1042/bj3580647
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