This paper describes structural characterization by NMR, MS and degradative studies of mucin glycans from normal human descending colon obtained freshly at autopsy. The saccharides were mainly based on core 3 (GlcNAcβ1-3GalNAc). Among the terminal saccharide determinants Sda/Cad-antigen-like structures were prominent, and Lewis x, sialyl Lewis x and sulphated Lewis x were found as minor components, whereas blood group H and A antigenic determinants were absent. The saccharides were markedly different from those of mucins from colon cancers or colon cancer cell lines analysed so far, in which cores 1 and 2 are prominent features, and in which various other terminal determinants have been found, but not Sda/Cad.
Sda-antigen-like structures carried on core 3 are prominent features of glycans from the mucin of normal human descending colon
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Calliope CAPON, Emmanuel MAES, Jean-Claude MICHALSKI, Hakon LEFFLER, Young S. KIM; Sda-antigen-like structures carried on core 3 are prominent features of glycans from the mucin of normal human descending colon. Biochem J 15 September 2001; 358 (3): 657–664. doi: https://doi.org/10.1042/bj3580657
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