The extra-enzymic function of cell-surface adenosine deaminase (ADA), an enzyme mainly localized in the cytosol but also found on the cell surface of monocytes, B cells and T cells, has lately been the subject of numerous studies. Cell-surface ADA is able to transduce co-stimulatory signals in T cells via its interaction with CD26, an integral membrane protein that acts as ADA-binding protein. The aim of the present study was to explore whether ADA—CD26 interaction plays a role in the adhesion of lymphocyte cells to human epithelial cells. To meet this aim, different lymphocyte cell lines (Jurkat and CEM T) expressing endogenous, or overexpressing human, CD26 protein were tested in adhesion assays to monolayers of colon adenocarcinoma human epithelial cells, Caco-2, which express high levels of cell-surface ADA. Interestingly, the adhesion of Jurkat and CEM T cells to a monolayer of Caco-2 cells was greatly dependent on CD26. An increase by 50% in the cell-to-cell adhesion was found in cells containing higher levels of CD26. Incubation with an anti-CD26 antibody raised against the ADA-binding site or with exogenous ADA resulted in a significant reduction (50–70%) of T-cell adhesion to monolayers of epithelial cells. The role of ADA—CD26 interaction in the lymphocyte—epithelial cell adhesion appears to be mediated by CD26 molecules that are not interacting with endogenous ADA (ADA-free CD26), since SKW6.4 (B cells) that express more cell-surface ADA showed lower adhesion than T cells. Adhesion stimulated by CD26 and ADA is mediated by T cell lymphocyte function-associated antigen. A role for ADA—CD26 interaction in cell-to-cell adhesion was confirmed further in integrin activation assays. FACS analysis revealed a higher expression of activated integrins on T cell lines in the presence of increasing amounts of exogenous ADA. Taken together, these results suggest that the ADA—CD26 interaction on the cell surface has a role in lymphocyte—epithelial cell adhesion.
Skip Nav Destination
Article navigation
January 2002
- PDF Icon PDF LinkFront Matter
Research Article|
January 08 2002
Regulation of epithelial and lymphocyte cell adhesion by adenosine deaminase–CD26 interaction
Silvia GINÉS
;
Silvia GINÉS
1
∗Department of Biochemistry and Molecular Biology, University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain
1To whom correspondence should be addressed, at the present address: Neurogenetics Unit, Massachusetts General Hospital East, Bldg 149, 13th Street, Charlestown, MA 02129, U.S.A. (e-mail gines@helix.mgh.harvard.edu).
Search for other works by this author on:
Marta MARIÑO
;
Marta MARIÑO
∗Department of Biochemistry and Molecular Biology, University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain
Search for other works by this author on:
Josefa MALLOL
;
Josefa MALLOL
∗Department of Biochemistry and Molecular Biology, University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain
Search for other works by this author on:
Enric I. CANELA
;
Enric I. CANELA
∗Department of Biochemistry and Molecular Biology, University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain
Search for other works by this author on:
Chikao MORIMOTO
;
Chikao MORIMOTO
†Division of Tumor Biology, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, U.S.A.
‡The AIDS Research Center, National Institute of Infectious Diseases, Tokyo 162-0052, Japan
Search for other works by this author on:
Christian CALLEBAUT
;
Christian CALLEBAUT
§Department of Cellular Immunology, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris cedex 15, Paris, France
Search for other works by this author on:
Ara HOVANESSIAN
;
Ara HOVANESSIAN
§Department of Cellular Immunology, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris cedex 15, Paris, France
Search for other works by this author on:
Vicent CASADÓ
;
Vicent CASADÓ
∗Department of Biochemistry and Molecular Biology, University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain
Search for other works by this author on:
Carmen LLUIS
;
Carmen LLUIS
∗Department of Biochemistry and Molecular Biology, University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain
Search for other works by this author on:
Rafael FRANCO
Rafael FRANCO
∗Department of Biochemistry and Molecular Biology, University of Barcelona, Martí i Franquès 1, 08028 Barcelona, Spain
Search for other works by this author on:
Biochem J (2002) 361 (2): 203–209.
Article history
Received:
March 21 2001
Revision Received:
September 19 2001
Accepted:
October 22 2001
Citation
Silvia GINÉS, Marta MARIÑO, Josefa MALLOL, Enric I. CANELA, Chikao MORIMOTO, Christian CALLEBAUT, Ara HOVANESSIAN, Vicent CASADÓ, Carmen LLUIS, Rafael FRANCO; Regulation of epithelial and lymphocyte cell adhesion by adenosine deaminase–CD26 interaction. Biochem J 15 January 2002; 361 (2): 203–209. doi: https://doi.org/10.1042/bj3610203
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
14
Views
0
Citations
Cited By
Related Articles
Perforin gene expression in T lymphocytes correlates with disease activity in immunoglobulin A nephropathy
Clin Sci (Lond) (April,1992)
Natural killer cells and T lymphocytes in pregnancy and pre-eclampsia
Clin Sci (Lond) (December,2017)
Integrins and Disease
Clin Sci (Lond) (December,1996)
Telomere diminution as a cause of immune failure in old age: an unfashionable demurral
Biochem Soc Trans (February,2000)