Previous studies indicated during replication of plasmids derived from bacteriophage λ (the so-called λ plasmids), that, once assembled, replication complex can be inherited by one of the two daughter plasmid copies after each replication round, and may function in subsequent replication rounds. It seems that similar processes occur during replication of other DNA molecules, including chromosomes of the yeast Saccharomyces cerevisiae. However, apart from some suggestions based on genetic experiments, composition of the λ heritable replication complex remains unknown. In amino acid-starved Escherichia coli relA mutants, replication of λ plasmid DNA is carried out exclusively by the heritable replication complex as assembly of new complexes is impaired due to inhibition of protein synthesis. Here, using a procedure based on in vivo cross-linking, cell lysis, immunoprecipitation with specific sera, de-cross-linking and PCR analysis, we demonstrate that the λ heritable replication complex consists of O, P, DnaB and, perhaps surprisingly, DnaK proteins.
Research Article| June 15 2002
Composition of the λ plasmid heritable replicationcomplex
Grzegorz WĘGRZYN 1
∗Department of Molecular Biology, University of Gdańsk, Kładki 24, 80-822 Gdańsk, Poland
‡Institute of Oceanology, Polish Academy of Sciences, Św. Wojciecha 5, 81-347 Gdynia, Poland
1To whom correspondence should be addressed, at the Department of Molecular Biology, University of Gdańsk (e-mail email@example.com).
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Katarzyna POTRYKUS, Sylwia BARAŃSKA, Alicja WĘGRZYN, Grzegorz WĘGRZYN; Composition of the λ plasmid heritable replicationcomplex. Biochem J 15 June 2002; 364 (3): 857–862. doi: https://doi.org/10.1042/bj20011488
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