Zearalenone (ZEN) is converted into a far less oestrogenic product by incubation with Clonostachys rosea IFO 7063. An alkaline hydrolase responsible for the detoxification was purified to homogeneity from the fungus by a combination of salt precipitation and column chromatography methods. The purified enzyme was homodimeric with a subunit molecular mass of 30kDa and contained an intra-subunit disulphide bridge. On the basis of the internal peptide sequences of the purified protein, we cloned the entire coding region of the gene (designated as zhd101) by PCR techniques. The ZEN degradation activity was detected in heterologous hosts (Schizosaccharomyces pombe and Escherichia coli) carrying the cloned gene. Zhd101 could be a promising genetic resource for in planta detoxification of the mycotoxin in important crops.
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Research Article|
July 01 2002
A novel lactonohydrolase responsible for the detoxification of zearalenone: enzyme purification and gene cloning
Naoko TAKAHASHI-ANDO;
Naoko TAKAHASHI-ANDO
∗Laboratory for Remediation Research, Plant Science Centre, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
†Microbial Toxicology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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Makoto KIMURA;
Makoto KIMURA
1
∗Laboratory for Remediation Research, Plant Science Centre, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
†Microbial Toxicology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
1To whom correspondence should be addressed (e-mail [email protected]).
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Hideaki KAKEYA;
Hideaki KAKEYA
‡Antibiotics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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Hiroyuki OSADA;
Hiroyuki OSADA
‡Antibiotics Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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Isamu YAMAGUCHI
Isamu YAMAGUCHI
∗Laboratory for Remediation Research, Plant Science Centre, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
†Microbial Toxicology Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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Publisher: Portland Press Ltd
Received:
March 22 2002
Revision Received:
April 22 2002
Accepted:
April 26 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 365 (1): 1–6.
Article history
Received:
March 22 2002
Revision Received:
April 22 2002
Accepted:
April 26 2002
Citation
Naoko TAKAHASHI-ANDO, Makoto KIMURA, Hideaki KAKEYA, Hiroyuki OSADA, Isamu YAMAGUCHI; A novel lactonohydrolase responsible for the detoxification of zearalenone: enzyme purification and gene cloning. Biochem J 1 July 2002; 365 (1): 1–6. doi: https://doi.org/10.1042/bj20020450
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