A Ca2+/calmodulin (CaM)-binding protein kinase from rice (Oryza sativa), OsCBK, has been characterized that lacks Ca2+-binding EF hands and has Ca2+/CaM-independent autophosphorylation and substrate-phosphorylation activity. OsCBK has all 11 subdomains of a kinase catalytic domain and a putative CaM-binding domain, and shares high identity with Ca2+-dependent-protein-kinase ('CDPK')-related protein kinases in plants. OsCBK bound CaM in a Ca2+-dependent manner as previously reported for Ca2+/calmodulin-dependent protein kinases in animals, but autophosphorylation and phosphorylation of histone IIIs were Ca2+/CaM-independent. Surface plasmon resonance analysis showed that OsCBK specifically bound CaM with high affinity (KD = 30nM). Capillary electrophoresis showed that phosphorylation of OsCBK occurred on serine and threonine residues. These data show that OsCBK is a serine/threonine protein kinase that binds Ca2+/CaM, but whose enzymic activity is independent of Ca2+/CaM. In situ hybridization showed that OsCBK is expressed in reproductive and vegetative tissues of rice and shows temporal and spatial changes during plant growth and development. OsCBK is highly expressed in zones of cell division and it is particularly abundant in sporogenous cells of the anther at meiosis.
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November 2002
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Research Article|
November 15 2002
Molecular and biochemical characterization of a calcium/calmodulin-binding protein kinase from rice Available to Purchase
Lei ZHANG;
Lei ZHANG
∗The Key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China,
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Bi-Feng LIU;
Bi-Feng LIU
∗The Key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China,
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Shuping LIANG;
Shuping LIANG
∗The Key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China,
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Russell L. JONES;
Russell L. JONES
†Department of Plant and Microbial Biology, University of California at Berkeley, CA 94720, U.S.A.
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Ying-Tang LU
Ying-Tang LU
1
∗The Key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan 430072, China,
1To whom correspondence should be addressed (e-mail [email protected]).
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Publisher: Portland Press Ltd
Received:
May 15 2002
Revision Received:
July 29 2002
Accepted:
August 02 2002
Accepted Manuscript online:
August 02 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2002
2002
Biochem J (2002) 368 (1): 145–157.
Article history
Received:
May 15 2002
Revision Received:
July 29 2002
Accepted:
August 02 2002
Accepted Manuscript online:
August 02 2002
Citation
Lei ZHANG, Bi-Feng LIU, Shuping LIANG, Russell L. JONES, Ying-Tang LU; Molecular and biochemical characterization of a calcium/calmodulin-binding protein kinase from rice. Biochem J 15 November 2002; 368 (1): 145–157. doi: https://doi.org/10.1042/bj20020780
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