Intracellular parasitic protozoans of the genus Leishmania depend for their survival on the elaboration of enzymic and other mechanisms for evading toxic free-radical damage inflicted by their phagocytic macrophage host. One such mechanism may involve superoxide dismutase (SOD), which detoxifies reactive superoxide radicals produced by activated macrophages, but the role of this enzyme in parasite survival has not yet been demonstrated. We have cloned a SOD gene from L. tropica and generated SOD-deficient parasites by expressing the corresponding antisense RNA from an episomal vector. Such parasites have enhanced sensitivity to menadione and hydrogen peroxide in axenic culture, and a markedly reduced survival in mouse macrophages. These results indicate that SOD is a major determinant of intracellular survival of Leishmania.
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February 2003
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Research Article|
February 01 2003
Role of superoxide dismutase in survival of Leishmania within the macrophage Available to Purchase
Sanjay GHOSH;
Sanjay GHOSH
Genetic Engineering laboratory, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Calcutta 700032, India
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Srikanta GOSWAMI;
Srikanta GOSWAMI
Genetic Engineering laboratory, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Calcutta 700032, India
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Samit ADHYA
Samit ADHYA
1
Genetic Engineering laboratory, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick Road, Calcutta 700032, India
1To whom correspondence should be addressed (e-mail [email protected]).
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Publisher: Portland Press Ltd
Received:
October 29 2002
Revision Received:
November 11 2002
Accepted:
December 02 2002
Accepted Manuscript online:
December 02 2002
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2003
2003
Biochem J (2003) 369 (3): 447–452.
Article history
Received:
October 29 2002
Revision Received:
November 11 2002
Accepted:
December 02 2002
Accepted Manuscript online:
December 02 2002
Citation
Sanjay GHOSH, Srikanta GOSWAMI, Samit ADHYA; Role of superoxide dismutase in survival of Leishmania within the macrophage. Biochem J 1 February 2003; 369 (3): 447–452. doi: https://doi.org/10.1042/bj20021684
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