Members of the ABC (ATP-binding cassette) transporter super-family are emerging to be involved in lipid transport. In the present study, we studied the organization of phospholipids in the plasma membrane of EPG85-257 human gastric carcinoma cells overexpressing BCRP (breast cancer resistance protein, ABCG-2), a half-size transporter belonging to the ABCG subfamily. A significantly increased plasma membrane association of the PS (phosphatidylserine)-binding probe FITC–Annexin V in comparison with control cells was observed. Treatment of BCRP-overexpressing cells with the inhibitor Tryprostatin A decreased FITC–Annexin V binding almost to the control level. This suggests an enhanced exposure of PS in BCRP-overexpressing cells, which is dependent on functional BCRP. A role of BCRP in the transverse distribution of lipids in the plasma membrane is supported by the increased outward transport of the lipid analogue C6-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-PS in BCRP-overexpressing EPG85-257 cells and MCF-7 human breast cancer cells. As shown for BCRP-overexpressing EPG85-257 cells, enhanced outward redistribution of the lipid analogue is inhibited by Tryprostatin A as well as by reduction of BCRP expression on transfection with an anti-BCRP-ribozyme. We also observed an enhanced outward transport of C6-N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-phosphatidylcholine in BCRP-overexpressing EPG85-257 cells, suggesting that the influence of BCRP on transverse lipid organization is not highly specific.
Skip Nav Destination
Close
Article navigation
December 2003
- PDF Icon PDF LinkFront Matter
Research Article|
December 01 2003
Enhanced exposure of phosphatidylserine in human gastric carcinoma cells overexpressing the half-size ABC transporter BCRP (ABCG2)
Holger WOEHLECKE
;
Holger WOEHLECKE
*Institute of Biology, Humboldt University Berlin, Invalidenstr. 43, 10115 Berlin, Germany
Search for other works by this author on:
Antje POHL
;
Antje POHL
1
*Institute of Biology, Humboldt University Berlin, Invalidenstr. 43, 10115 Berlin, Germany
Search for other works by this author on:
Nele ALDER-BAERENS
;
Nele ALDER-BAERENS
*Institute of Biology, Humboldt University Berlin, Invalidenstr. 43, 10115 Berlin, Germany
Search for other works by this author on:
Hermann LAGE
;
Hermann LAGE
†Institute of Pathology, Charité, Humboldt University Berlin, Schumannstr. 20/21, 10117 Berlin, Germany
Search for other works by this author on:
Andreas HERRMANN
Andreas HERRMANN
2
*Institute of Biology, Humboldt University Berlin, Invalidenstr. 43, 10115 Berlin, Germany
2To whom correspondence should be addressed (e-mail andreas.herrmann@rz.hu-berlin.de).
Search for other works by this author on:
Biochem J (2003) 376 (2): 489–495.
Article history
Received:
June 16 2003
Revision Received:
August 26 2003
Accepted:
August 28 2003
Accepted Manuscript online:
August 28 2003
Citation
Holger WOEHLECKE, Antje POHL, Nele ALDER-BAERENS, Hermann LAGE, Andreas HERRMANN; Enhanced exposure of phosphatidylserine in human gastric carcinoma cells overexpressing the half-size ABC transporter BCRP (ABCG2). Biochem J 1 December 2003; 376 (2): 489–495. doi: https://doi.org/10.1042/bj20030886
Download citation file:
Close
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Related Articles
Transport of phosphatidylserine via MDR1 (multidrug resistance 1)P-glycoprotein in a human gastric carcinoma cell line
Biochem J (July,2002)
The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane
Biochem J (December,2016)
Cloning, characterization and tissue distribution of the rat ATP-binding cassette (ABC) transporter ABC2/ABCA2
Biochem J (September,2000)
Substrate trajectory through phospholipid-transporting P4-ATPases
Biochem Soc Trans (September,2014)