The rat Hst70 gene and its mouse counterpart Hsp70.2 are expressed specifically in pachytene primary spermatocytes and spermatids. Here we demonstrate that a 165 bp fragment of the Hst70 gene promoter, containing the T1 transcription start site region, entire exon 1 and 42 bp 5´ region of the intron, is sufficient to drive testis-specific expression of the chloramphenicol acetyltransferase reporter gene in transgenic mice with the same developmentally regulated pattern as the endogenous Hsp70.2 gene. We show further that high-level tissue-specific gene expression requires additional sequences localized upstream of the T2 transcription start site. Electrophoretic mobility-shift assay analysis revealed that only testes of juvenile rats, when Hst70 gene expression is repressed, contain proteins that specifically bind to the Oct (octamer) sequence localized directly downstream of the T1 site.
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Research Article|
May 01 2004
Location of promoter elements necessary and sufficient to direct testis-specific expression of the Hst70/Hsp70.2 gene
Dorota ŚCIEGLIŃSKA;
Dorota ŚCIEGLIŃSKA
Department of Tumour Biology, Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Wybrzeże Armii Krajowej 15, 44-101 Gliwice, Poland
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Natallia VYDRA;
Natallia VYDRA
Department of Tumour Biology, Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Wybrzeże Armii Krajowej 15, 44-101 Gliwice, Poland
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Zdzisław KRAWCZYK;
Zdzisław KRAWCZYK
Department of Tumour Biology, Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Wybrzeże Armii Krajowej 15, 44-101 Gliwice, Poland
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Wiesława WIDŁAK
Wiesława WIDŁAK
1
Department of Tumour Biology, Centre of Oncology, Maria Skłodowska-Curie Memorial Institute, Wybrzeże Armii Krajowej 15, 44-101 Gliwice, Poland
1To whom correspondence should be addressed (e-mail [email protected]).
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Publisher: Portland Press Ltd
Received:
December 01 2003
Revision Received:
January 29 2004
Accepted:
February 06 2004
Accepted Manuscript online:
February 06 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2004
2004
Biochem J (2004) 379 (3): 739–747.
Article history
Received:
December 01 2003
Revision Received:
January 29 2004
Accepted:
February 06 2004
Accepted Manuscript online:
February 06 2004
Citation
Dorota ŚCIEGLIŃSKA, Natallia VYDRA, Zdzisław KRAWCZYK, Wiesława WIDŁAK; Location of promoter elements necessary and sufficient to direct testis-specific expression of the Hst70/Hsp70.2 gene. Biochem J 1 May 2004; 379 (3): 739–747. doi: https://doi.org/10.1042/bj20031842
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