We have described recently the purification and cloning of PP2A (protein phosphatase 2A) leucine carboxylmethyltransferase. We studied the purification of a PP2A-specific methylesterase that co-purifies with PP2A and found that it is tightly associated with an inactive dimeric or trimeric form of PP2A. These inactive enzyme forms could be reactivated as Ser/Thr phosphatase by PTPA (phosphotyrosyl phosphatase activator of PP2A). PTPA was described previously by our group as a protein that stimulates the in vitro phosphotyrosyl phosphatase activity of PP2A; however, PP2A-specific methyltransferase could not bring about the activation. The PTPA activation could be distinguished from the Mn2+ stimulation observed with some inactive forms of PP2A, also found associated with PME-1 (phosphatase methylesterase 1). We discuss a potential new function for PME-1 as an enzyme that stabilizes an inactivated pool of PP2A.
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May 15 2004
An inactive protein phosphatase 2A population is associated with methylesterase and can be re-activated by the phosphotyrosyl phosphatase activator Available to Purchase
Sari LONGIN;
Sari LONGIN
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Jan JORDENS;
Jan JORDENS
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Ellen MARTENS;
Ellen MARTENS
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Ilse STEVENS;
Ilse STEVENS
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Veerle JANSSENS;
Veerle JANSSENS
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Evelien RONDELEZ;
Evelien RONDELEZ
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Ivo DE BAERE;
Ivo DE BAERE
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Rita DERUA;
Rita DERUA
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Etienne WAELKENS;
Etienne WAELKENS
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Jozef GORIS;
Jozef GORIS
1
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
1To whom correspondence should be addressed (e-mail [email protected]).
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Christine VAN HOOF
Christine VAN HOOF
Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium
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Publisher: Portland Press Ltd
Received:
October 28 2003
Revision Received:
January 21 2004
Accepted:
January 28 2004
Accepted Manuscript online:
January 28 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2004
2004
Biochem J (2004) 380 (1): 111–119.
Article history
Received:
October 28 2003
Revision Received:
January 21 2004
Accepted:
January 28 2004
Accepted Manuscript online:
January 28 2004
Citation
Sari LONGIN, Jan JORDENS, Ellen MARTENS, Ilse STEVENS, Veerle JANSSENS, Evelien RONDELEZ, Ivo DE BAERE, Rita DERUA, Etienne WAELKENS, Jozef GORIS, Christine VAN HOOF; An inactive protein phosphatase 2A population is associated with methylesterase and can be re-activated by the phosphotyrosyl phosphatase activator. Biochem J 15 May 2004; 380 (1): 111–119. doi: https://doi.org/10.1042/bj20031643
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