Exosomes are small vesicles secreted from multivesicular bodies, which are able to stimulate the immune system leading to tumour cell eradication. We have analysed lipids of exosomes secreted either upon stimulation from rat mast cells (RBL-2H3 cells), or constitutively from human dendritic cells. As compared with parent cells, exosomes displayed an enrichment in sphingomyelin, but not in cholesterol. Phosphatidylcholine content was decreased, but an enrichment was noted in disaturated molecular species as in phosphatidylethanolamines. Lyso(bis)phosphatidic acid was not enriched in exosomes as compared with cells. Fluorescence anisotropy demonstrated an increase in exosome-membrane rigidity from pH 5 to 7, suggesting their membrane reorganization between the acidic multivesicular body compartment and the neutral outer cell medium. NMR analysis established a bilayer organization of exosome membrane, and ESR studies using 16-doxyl stearic acid demonstrated a higher flip-flop of lipids between the two leaflets as compared with plasma membrane. In addition, the exosome membrane exhibited no asymmetrical distribution of phosphatidylethanolamines. Therefore exosome membrane displays a similar content of the major phospholipids and cholesterol, and is organized as a lipid bilayer with a random distribution of phosphatidylethanolamines. In addition, we observed tight lipid packing at neutral pH and a rapid flip-flop between the two leaflets of exosome membranes. These parameters could be used as a hallmark of exosomes.
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May 15 2004
Mast cell- and dendritic cell-derived exosomes display a specific lipid composition and an unusual membrane organization Available to Purchase
Karine LAULAGNIER;
Karine LAULAGNIER
*INSERM U563, Département “Lipoprotéines et Médiateurs Lipidiques”, CPTP, Place du Dr Baylac, Hôpital Purpan, 31059 Toulouse Cedex 03, France
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Claude MOTTA;
Claude MOTTA
†CHU Pontchaillou, Laboratoire de Biochimie, 2 rue Henri Le Guilloux, 35000 Rennes, France
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Safouane HAMDI;
Safouane HAMDI
*INSERM U563, Département “Lipoprotéines et Médiateurs Lipidiques”, CPTP, Place du Dr Baylac, Hôpital Purpan, 31059 Toulouse Cedex 03, France
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Sébastien ROY;
Sébastien ROY
*INSERM U563, Département “Lipoprotéines et Médiateurs Lipidiques”, CPTP, Place du Dr Baylac, Hôpital Purpan, 31059 Toulouse Cedex 03, France
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Florence FAUVELLE;
Florence FAUVELLE
‡Centre de Recherche du service de Santé des Armées (CRSSA), Avenue du Grésivaudan, 38700 La Tronche, France
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Jean-François PAGEAUX;
Jean-François PAGEAUX
§INSERM U585 “Physiopathologie des lipides et des membranes”, INSA, 20 Avenue Albert Einstein, 69621 Villeurbanne, France
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Toshihide KOBAYASHI;
Toshihide KOBAYASHI
§INSERM U585 “Physiopathologie des lipides et des membranes”, INSA, 20 Avenue Albert Einstein, 69621 Villeurbanne, France
‖Riken Institute, Hirosawa, Wako, Saitama 351-0918, Japan
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Jean-Pierre SALLES;
Jean-Pierre SALLES
*INSERM U563, Département “Lipoprotéines et Médiateurs Lipidiques”, CPTP, Place du Dr Baylac, Hôpital Purpan, 31059 Toulouse Cedex 03, France
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Bertrand PERRET;
Bertrand PERRET
*INSERM U563, Département “Lipoprotéines et Médiateurs Lipidiques”, CPTP, Place du Dr Baylac, Hôpital Purpan, 31059 Toulouse Cedex 03, France
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Christian BONNEROT;
Christian BONNEROT
¶INSERM U520 “Biologie Cellulaire de l'Immunité anti-tumorale”, Institut Curie, 26 rue d'Ulm, 75248 Paris, France
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Michel RECORD
Michel RECORD
1
*INSERM U563, Département “Lipoprotéines et Médiateurs Lipidiques”, CPTP, Place du Dr Baylac, Hôpital Purpan, 31059 Toulouse Cedex 03, France
1To whom correspondence should be addressed (e-mail [email protected]).
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Publisher: Portland Press Ltd
Received:
October 20 2003
Revision Received:
February 12 2004
Accepted:
February 13 2004
Accepted Manuscript online:
February 13 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London ©2004
2004
Biochem J (2004) 380 (1): 161–171.
Article history
Received:
October 20 2003
Revision Received:
February 12 2004
Accepted:
February 13 2004
Accepted Manuscript online:
February 13 2004
Citation
Karine LAULAGNIER, Claude MOTTA, Safouane HAMDI, Sébastien ROY, Florence FAUVELLE, Jean-François PAGEAUX, Toshihide KOBAYASHI, Jean-Pierre SALLES, Bertrand PERRET, Christian BONNEROT, Michel RECORD; Mast cell- and dendritic cell-derived exosomes display a specific lipid composition and an unusual membrane organization. Biochem J 15 May 2004; 380 (1): 161–171. doi: https://doi.org/10.1042/bj20031594
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