Nucling is a novel apoptosis-associated molecule, which is involved with cytochrome c/Apaf-1/caspase-9 apoptosome induction following pro-apoptotic stress. In the present study, we show first that Nucling is able to interact with galectin-3. Galectin-3 is known to participate in many biological processes, including apoptotic cell death. Nucling was found to down-regulate the expression level of galectin-3 mRNA/protein. Nucling-deficient cells, in which galectin-3 expression is up-regulated, appeared to be resistant to some forms of pro-apoptotic stress as compared with wild-type cells. In addition, the preputial gland from Nucling-deficient mice expressed a significant level of galectin-3 and exhibited a high incidence of inflammatory lesions, indicating that Nucling plays a crucial role in the homoeostasis of this gland by interacting with the galectin-3 molecule and regulating the expression level of galectin-3. Up-regulation of galectin-3 was also observed in the heart, kidney, lung, testis and ovary of the Nucling-deficient mice. In order to confirm the functional interaction between Nucling and galectin-3, a well-documented candidate for the mediator of galectin-3 expression, NF-κB (nuclear factor κB), was investigated as well. Nucling was shown to interfere with NF-κB activation via the nuclear translocation process of NF-κB/p65, thus inhibiting the expression of galectin-3. Taken together, we propose that Nucling mediates apoptosis by interacting and inhibiting expression of galectin-3.
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Research Article|
May 15 2004
Nucling mediates apoptosis by inhibiting expression of galectin-3 through interference with nuclear factor kappaB signalling
Li LIU
;
Li LIU
*The Institute for Enzyme Research, The University of Tokushima, 3-18-15 Kuramoto-Cho, Tokushima, 770-8503, Japan
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Takashi SAKAI
;
Takashi SAKAI
*The Institute for Enzyme Research, The University of Tokushima, 3-18-15 Kuramoto-Cho, Tokushima, 770-8503, Japan
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Nobuya SANO
;
Nobuya SANO
†University Hospital, The University of Tokushima, 3–18–15 Kuramoto-Cho, Tokushima, 770-8503, Japan
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Kiyoshi FUKUI
Kiyoshi FUKUI
1
*The Institute for Enzyme Research, The University of Tokushima, 3-18-15 Kuramoto-Cho, Tokushima, 770-8503, Japan
1To whom correspondence should be addressed (e-mail kiyo@ier.tokushima-u.ac.jp).
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Biochem J (2004) 380 (1): 31–41.
Article history
Received:
August 26 2003
Revision Received:
December 16 2003
Accepted:
February 13 2004
Accepted Manuscript online:
February 13 2004
Citation
Li LIU, Takashi SAKAI, Nobuya SANO, Kiyoshi FUKUI; Nucling mediates apoptosis by inhibiting expression of galectin-3 through interference with nuclear factor kappaB signalling. Biochem J 15 May 2004; 380 (1): 31–41. doi: https://doi.org/10.1042/bj20031300
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