Human TFR2 (transferrin receptor 2) is a membrane-bound protein homologous with TFR1. High levels of TFR2 mRNA were found mainly in the liver and, to a lesser extent, in erythroid precursors. However, although the presence of the TFR2 protein in hepatic cells has been confirmed in several studies, evidence is lacking about the presence of the TFR2 protein in normal erythroid cells. Using two anti-TFR2 monoclonal antibodies, G/14C2 and G/14E8, we have provided evidence that TFR2 protein is not expressed in normal erythroid cells at any stage of differentiation, from undifferentiated CD34+ cells to mature orthochromatic erythroblasts. In contrast, erythroleukaemic cells (K562 cells) exhibited a high level of expression of TFR2 at both the mRNA and the protein level. We can therefore conclude that an elevated expression of TFR2 protein is observed in leukaemic cells, but not in normal erythroblasts. The implications of this observation for the understanding of the phenotypic features of haemochromatosis due to mutation of the TFR2 gene are discussed.
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August 2004
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Research Article|
July 27 2004
Transferrin receptor 2 protein is not expressed in normal erythroid cells
Alessia CALZOLARI;
Alessia CALZOLARI
*Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
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Silvia DEAGLIO;
Silvia DEAGLIO
†Laboratory of Immunogenetics, Department of Genetics, Biology, and Biochemistry and CeRMS, University of Turin Medical School, via Santena 19, 10125 Turin, Italy
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Nadia Maria SPOSI;
Nadia Maria SPOSI
*Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
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Eleonora PETRUCCI;
Eleonora PETRUCCI
*Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
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Ornella MORSILLI;
Ornella MORSILLI
*Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
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Marco GABBIANELLI;
Marco GABBIANELLI
*Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
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Fabio MALAVASI;
Fabio MALAVASI
*Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
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Cesare PESCHLE;
Cesare PESCHLE
*Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
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Ugo TESTA
Ugo TESTA
1
*Department of Hematology, Oncology and Molecular Medicine, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy
1To whom correspondence should be addressed (e-mail u.testa@iss.it).
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Biochem J (2004) 381 (3): 629–634.
Article history
Received:
February 11 2004
Revision Received:
March 31 2004
Accepted:
April 15 2004
Accepted Manuscript online:
April 15 2004
Citation
Alessia CALZOLARI, Silvia DEAGLIO, Nadia Maria SPOSI, Eleonora PETRUCCI, Ornella MORSILLI, Marco GABBIANELLI, Fabio MALAVASI, Cesare PESCHLE, Ugo TESTA; Transferrin receptor 2 protein is not expressed in normal erythroid cells. Biochem J 1 August 2004; 381 (3): 629–634. doi: https://doi.org/10.1042/BJ20040230
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