Septins are a cytosolic GTP-binding protein family first characterized in yeast, but gaining increasing recognition as critical protagonists in higher eukaryotic cellular events. Mammalian septins have been associated with cytokinesis and exocytosis, along with contributing to the development of neurological disorders. Ten different septins, divided into four groups, have been identified in mammals, and individual septins are capable of interacting with each other to form macromolecular complexes. The present study characterizes the structural requirements for human septin–septin interactions using a yeast two-hybrid system. We focus on three septins that are highly expressed in platelets and neurons, SEPT4 [previously designated H5, CDCrel-2 (cell-division-control-related-2), PNUTL2], SEPT5 (CDCrel-1, PNUTL1) and SEPT8 (KIAA0202). Each of these three septins contains a characteristic domain structure consisting of unique N- and C-termini, and a central core domain conserved among the family of proteins. The yeast two-hybrid system yielded data consistent with a model where each of the three septins can interact with itself (homotypic assembly) or with one of the other septins (heterotypic assembly). For SEPT5 and SEPT8, the results illustrate a model whereby heterotypic septin assembly is dependent on the conserved central core domain and homotypic interactions require the N- and C-termini of each protein. We also characterized a model in which the proper cellular localization of SEPT5 and SEPT8 requires concomitant expression of both proteins. Co-transfection of SEPT5 and SEPT8 results in both proteins targeted to a vesicular-like location. Therefore the cellular repertoire of human septins has an impact on function by targeting septin macromolecular complexes to specific cellular locations.
Skip Nav Destination
Follow us on Twitter @Biochem_Journal
Article navigation
September 2004
-
Cover Image
Cover Image
- PDF Icon PDF LinkFront Matter
- PDF Icon PDF LinkTable of Contents
- PDF Icon PDF LinkEditorial Board
Research Article|
September 07 2004
Human septin–septin interactions as a prerequisite for targeting septin complexes in the cytosol
Constantino MARTÍNEZ;
Constantino MARTÍNEZ
*The Roon Research Center for Arteriosclerosis and Thrombosis, Division of Experimental Hemostasis and Thrombosis, Department of Molecular and Experimental Medicine The Scripps Research Institute, La Jolla, CA 92037, U.S.A.
Search for other works by this author on:
Miguel A. SANJUAN;
Miguel A. SANJUAN
†Johnson & Johnson Pharmaceutical Research & Development, L.L.C., La Jolla, CA 92121, U.S.A.
Search for other works by this author on:
Judith A. DENT;
Judith A. DENT
*The Roon Research Center for Arteriosclerosis and Thrombosis, Division of Experimental Hemostasis and Thrombosis, Department of Molecular and Experimental Medicine The Scripps Research Institute, La Jolla, CA 92037, U.S.A.
Search for other works by this author on:
Lars KARLSSON;
Lars KARLSSON
†Johnson & Johnson Pharmaceutical Research & Development, L.L.C., La Jolla, CA 92121, U.S.A.
Search for other works by this author on:
Jerry WARE
Jerry WARE
1
*The Roon Research Center for Arteriosclerosis and Thrombosis, Division of Experimental Hemostasis and Thrombosis, Department of Molecular and Experimental Medicine The Scripps Research Institute, La Jolla, CA 92037, U.S.A.
1To whom correspondence should be addressed at the present address: Department of Physiology and Biophysics, #505, University of Arkansas for Medical Sciences, Little Rock, AR 72223, U.S.A. (email [email protected]).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
March 08 2004
Revision Received:
June 03 2004
Accepted:
June 23 2004
Accepted Manuscript online:
June 23 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2004
Biochem J (2004) 382 (3): 783–791.
Article history
Received:
March 08 2004
Revision Received:
June 03 2004
Accepted:
June 23 2004
Accepted Manuscript online:
June 23 2004
Citation
Constantino MARTÍNEZ, Miguel A. SANJUAN, Judith A. DENT, Lars KARLSSON, Jerry WARE; Human septin–septin interactions as a prerequisite for targeting septin complexes in the cytosol. Biochem J 15 September 2004; 382 (3): 783–791. doi: https://doi.org/10.1042/BJ20040372
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Follow us on Twitter @Biochem_Journal
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() View past webinars > |