The yeast SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) protein Ykt6 was shown to mediate palmitoylation of the fusion factor Vac8 in a reaction essential for the fusion of vacuoles. Here I present evidence that hYkt6 (human Ykt6) has self-palmitoylating activity. Incubation of recombinant hYkt6 with [3H]Pal-CoA ([3H]palmitoyl-CoA) leads to covalent attachment of palmitate to C-terminal cysteine residues. The N-terminal domain of human Ykt6 contains a Pal-CoA binding site and is required for the reaction.
Research Article| November 23 2004
The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues
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Michael VEIT; The human SNARE protein Ykt6 mediates its own palmitoylation at C-terminal cysteine residues. Biochem J 1 December 2004; 384 (2): 233–237. doi: https://doi.org/10.1042/BJ20041474
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