We have cloned a gene, ZFF29 (zinc-finger protein of human fetal liver erythroid cells 29), from human fetal liver erythroid cells. Two types of mature mRNA were identified and designated ZFF29a and ZFF29b. In human genome the ZFF29 gene is on chromosome 9q, and the two forms are splice variants. There is a unique transcription start site, which predicts major mRNAs composed of 2485 bases for ZFF29a and 1801 bases for ZFF29b. The anticipated mRNAs were demonstrated in K562 cells, but not in any adult human tissues examined by Northern blotting. In the mouse, reverse transcription–PCR revealed that the ZFF29 mRNA is present in adult bone marrow and ovary at a higher level than in any other tissues examined. These findings suggest that ZFF29 proteins are expressed in embryonic/fetal erythroid tissues. The deduced polypeptide chains of ZFF29a and ZFF29b are composed of 306 and 350 amino acids respectively. A unique zinc-finger motif composed of two contiguous Cys2His2-type fingers is common to both forms of ZFF29. They are nuclear proteins and ZFF29b, but not ZFF29a, is an activator of erythroid gene promoters.
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December 2004
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Research Article|
December 07 2004
Molecular cloning and characterization of ZFF29: a protein containing a unique Cys2His2 zinc-finger motif
Haruhiko ASANO;
Haruhiko ASANO
1
*Department of Hematology, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya, Japan 466-8550
1To whom correspondence should be addressed (email [email protected]).
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Takashi MURATE;
Takashi MURATE
†Nagoya University School of Health Science, Daiko-minami, 1-1-20, Higashi-ku, Nagoya, Japan 461-8673
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Tomoki NAOE;
Tomoki NAOE
*Department of Hematology, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya, Japan 466-8550
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Hidehiko SAITO;
Hidehiko SAITO
2
*Department of Hematology, Nagoya University Graduate School of Medicine, Tsurumai-cho 65, Showa-ku, Nagoya, Japan 466-8550
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George STAMATOYANNOPOULOS
George STAMATOYANNOPOULOS
‡Division of Medical Genetics, University of Washington, Seattle, WA 98195-7720, U.S.A.
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Publisher: Portland Press Ltd
Received:
March 11 2004
Revision Received:
August 20 2004
Accepted:
September 02 2004
Accepted Manuscript online:
September 02 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2004
Biochem J (2004) 384 (3): 647–653.
Article history
Received:
March 11 2004
Revision Received:
August 20 2004
Accepted:
September 02 2004
Accepted Manuscript online:
September 02 2004
Citation
Haruhiko ASANO, Takashi MURATE, Tomoki NAOE, Hidehiko SAITO, George STAMATOYANNOPOULOS; Molecular cloning and characterization of ZFF29: a protein containing a unique Cys2His2 zinc-finger motif. Biochem J 15 December 2004; 384 (3): 647–653. doi: https://doi.org/10.1042/BJ20040394
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