We have investigated the role of the protein ubiquitous mitochondrial creatine kinase (uMtCK) in the formation and stabilization of inner and outer membrane contact sites. Using liver mitochondria isolated from transgenic mice, which, unlike control animals, express uMtCK in the liver, we found that the enzyme was associated with the mitochondrial membranes and, in addition, was located in membrane-coated matrix inclusions. In mitochondria isolated from uMtCK transgenic mice, the number of contact sites increased 3-fold compared with that observed in control mitochondria. Furthermore, uMtCK-containing mitochondria were more resistant to detergent-induced lysis than wild-type mitochondria. We conclude that octameric uMtCK induces the formation of mitochondrial contact sites, leading to membrane cross-linking and to an increased stability of the mitochondrial membrane architecture.
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January 2005
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Research Article|
January 07 2005
Octameric mitochondrial creatine kinase induces and stabilizes contact sites between the inner and outer membrane
Oliver SPEER;
Oliver SPEER
1
*Swiss Federal Institute of Technology, ETH-Zürich, Institute of Cell Biology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland
†Institute of Biomedicine, University of Helsinki, FIN-00014 Finland
1To whom correspondence should be addressed at the present address: Institute of Molecular Biology, Winterthurerstrasse 190, University of Zürich, CH-8053 Zürich, Switzerland (email Oliver.Speer@molbio.unizh.ch).
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Nils BÄCK;
Nils BÄCK
†Institute of Biomedicine, University of Helsinki, FIN-00014 Finland
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Tanja BUERKLEN;
Tanja BUERKLEN
*Swiss Federal Institute of Technology, ETH-Zürich, Institute of Cell Biology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland
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Dieter BRDICZKA;
Dieter BRDICZKA
‡Department of Life Sciences, University of Konstanz, D-78457 Konstanz, Germany
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Alan KORETSKY;
Alan KORETSKY
§Laboratory of Functional and Molecular Imaging, National Institutes of Neurological Disorders and Stroke NIH, Bethesda, MD 29892, U.S.A.
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Theo WALLIMANN;
Theo WALLIMANN
*Swiss Federal Institute of Technology, ETH-Zürich, Institute of Cell Biology, ETH-Hönggerberg, CH-8093 Zürich, Switzerland
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Ove ERIKSSON
Ove ERIKSSON
†Institute of Biomedicine, University of Helsinki, FIN-00014 Finland
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Biochem J (2005) 385 (2): 445–450.
Article history
Received:
March 10 2004
Revision Received:
July 20 2004
Accepted:
August 05 2004
Accepted Manuscript online:
August 05 2004
Citation
Oliver SPEER, Nils BÄCK, Tanja BUERKLEN, Dieter BRDICZKA, Alan KORETSKY, Theo WALLIMANN, Ove ERIKSSON; Octameric mitochondrial creatine kinase induces and stabilizes contact sites between the inner and outer membrane. Biochem J 15 January 2005; 385 (2): 445–450. doi: https://doi.org/10.1042/BJ20040386
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