We describe the characterization of a family 4 UDG1 (uracil DNA glycosylase) from the crenarchaeote Sulfolobus solfataricus. UDG1 is found to have a marked preference for substrates containing a G:U base pair over either A:U or single-stranded uracil-containing DNA substrates. UDG1 is found to interact with the sliding clamp PCNA (proliferating cell nuclear antigen), and does so by a conserved motif in the C-terminus of the protein. S. solfataricus has a heterotrimeric PCNA, and only one of the subunits, PCNA3, interacts with UDG1. We have been unable to detect any stimulation of UDG activity by PCNA, in contrast with the observed effects of PCNA on a number of DNA metabolic enzymes. However, analysis of the effects of Sulfolobus chromatin proteins on UDG1 leads us to propose a mechanistic basis for coupling UDG1 to the replication fork.
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Research Article|
April 26 2005
Characterization of an archaeal family 4 uracil DNA glycosylase and its interaction with PCNA and chromatin proteins Available to Purchase
Isabelle DIONNE;
Isabelle DIONNE
1Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, U.K.
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Stephen D. BELL
Stephen D. BELL
1
1Medical Research Council Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, U.K.
1To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
September 30 2004
Revision Received:
December 01 2004
Accepted:
December 10 2004
Accepted Manuscript online:
December 10 2004
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2005
Biochem J (2005) 387 (3): 859–863.
Article history
Received:
September 30 2004
Revision Received:
December 01 2004
Accepted:
December 10 2004
Accepted Manuscript online:
December 10 2004
Citation
Isabelle DIONNE, Stephen D. BELL; Characterization of an archaeal family 4 uracil DNA glycosylase and its interaction with PCNA and chromatin proteins. Biochem J 1 May 2005; 387 (3): 859–863. doi: https://doi.org/10.1042/BJ20041661
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