Mammalian Stau1 (Staufen1), a modular protein composed of several dsRBDs (double-stranded RNA-binding domains), is probably involved in mRNA localization. Although Stau1 is mostly described in association with the rough endoplasmic reticulum and ribosomes in the cytoplasm, recent studies suggest that it may transit through the nucleus/nucleolus. Using a sensitive yeast import assay, we show that Stau1 is actively imported into the nucleus through a newly identified bipartite nuclear localization signal. As in yeast, the bipartite nuclear localization signal is necessary for Stau1 nuclear import in mammalian cells. It is also required for Stau1 nucleolar trafficking. However, Stau1 nuclear transit seems to be regulated by mechanisms that involve cytoplasmic retention and/or facilitated nuclear export. Cytoplasmic retention is mainly achieved through the action of dsRBD3, with dsRBD2 playing a supporting role in this function. Similarly, dsRBD3, but not its RNA-binding activity, is critical for Stau1 nucleolar trafficking. The function of dsRBD3 is strengthened or stabilized by the presence of dsRBD4 but prevented by the interdomain between dsRBD2 and dsRBD3. Altogether, these results suggest that Stau1 nuclear trafficking is a highly regulated process involving several determinants. The presence of Stau1 in the nucleus/nucleolus suggests that it may be involved in ribonucleoprotein formation in the nucleus and/or in other nuclear functions not necessarily related to mRNA transport.
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December 12 2005
Staufen1 is imported into the nucleolus via a bipartite nuclear localization signal and several modulatory determinants Available to Purchase
Catherine Martel;
Catherine Martel
*Department of Biochemistry, University of Montreal, P.O. Box 6128, Station Centre Ville, Montreal, Canada H3C 3J7
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Paolo Macchi;
Paolo Macchi
†Center for Brain Research, Medical University of Vienna, Vienna, Austria
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Luc Furic;
Luc Furic
*Department of Biochemistry, University of Montreal, P.O. Box 6128, Station Centre Ville, Montreal, Canada H3C 3J7
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Michael A. Kiebler;
Michael A. Kiebler
†Center for Brain Research, Medical University of Vienna, Vienna, Austria
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Luc Desgroseillers
Luc Desgroseillers
1
*Department of Biochemistry, University of Montreal, P.O. Box 6128, Station Centre Ville, Montreal, Canada H3C 3J7
1To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
April 27 2005
Revision Received:
September 12 2005
Accepted:
September 15 2005
Accepted Manuscript online:
September 15 2005
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2006
Biochem J (2006) 393 (1): 245–254.
Article history
Received:
April 27 2005
Revision Received:
September 12 2005
Accepted:
September 15 2005
Accepted Manuscript online:
September 15 2005
Citation
Catherine Martel, Paolo Macchi, Luc Furic, Michael A. Kiebler, Luc Desgroseillers; Staufen1 is imported into the nucleolus via a bipartite nuclear localization signal and several modulatory determinants. Biochem J 1 January 2006; 393 (1): 245–254. doi: https://doi.org/10.1042/BJ20050694
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