Diabetes mellitus is associated with a high incidence and poor prognosis of cardiovascular disease. The aim of the present study was to examine the effect of relatively short-term (5 weeks) Type I diabetes on the left ventricle, the right ventricle and the vessel (vein) on the left ventricle of the myocardium at molecular level by FTIR (Fourier-transform infrared) microspectroscopy. The rats were categorized into two groups: control group (for the left ventricle myocardium, n=8; for the right ventricle myocardium, n=9; for the vein, n=9) and streptozotocin-induced diabetic group (for the left ventricle myocardium, n=7; for the right ventricle myocardium, n=9; for the vein, n=8). Two adjacent cross-sections of 9 μm thickness were taken from the ventricles of the hearts in two groups of rats by using a cryotome. The first sections were used for FTIR microspectroscopy measurements. The second serial sections were stained by haematoxylin/eosin for comparative purposes. Diabetes caused an increase in the content of lipids, an alteration in protein profile with a decrease in α-helix and an increase in β-sheet structure as well as an increase in glycogen and glycolipid contents in both ventricles and the vein. Additionally, the collagen content was found to be increased in the vein of the diabetic group. The present study demonstrated that diabetes-induced alterations in the rat heart can be detected by correlating the IR spectral changes with biochemical profiles in detail. The present study for the first time demonstrated the diabetes-induced alterations at molecular level in both ventricle myocardia and the veins in relatively short-term diabetes.
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August 2006
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Research Article|
July 13 2006
Early alterations in myocardia and vessels of the diabetic rat heart: an FTIR microspectroscopic study
Neslihan Toyran;
Neslihan Toyran
1
*Department of Biological Sciences, Middle East Technical University, 06531 Ankara, Turkey
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Peter Lasch;
Peter Lasch
†P25-Biomedical Spectroscopy, Robert Koch-Institut, Nordufer 20, D-13353 Berlin, Germany
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Dieter Naumann;
Dieter Naumann
†P25-Biomedical Spectroscopy, Robert Koch-Institut, Nordufer 20, D-13353 Berlin, Germany
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Belma Turan;
Belma Turan
‡Department of Biophysics, Faculty of Medicine, Ankara University, 06100 Ankara, Turkey
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Feride Severcan
Feride Severcan
2
*Department of Biological Sciences, Middle East Technical University, 06531 Ankara, Turkey
2To whom correspondence should be addressed (email feride@metu.edu.tr).
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Biochem J (2006) 397 (3): 427–436.
Article history
Received:
January 30 2006
Revision Received:
April 05 2006
Accepted:
April 12 2006
Accepted Manuscript online:
April 12 2006
Citation
Neslihan Toyran, Peter Lasch, Dieter Naumann, Belma Turan, Feride Severcan; Early alterations in myocardia and vessels of the diabetic rat heart: an FTIR microspectroscopic study. Biochem J 1 August 2006; 397 (3): 427–436. doi: https://doi.org/10.1042/BJ20060171
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