Synthesis of VLCFAs (very long chain fatty acids) and biosynthesis of DHS (dihydrosphingosine) both are of vital importance for Saccharomyces cerevisiae. The bulk of VLCFAs and DHS are used for ceramide synthesis by the Lag1p (longevity-assurance gene 1)/Lac1p (longevity-assurance gene cognate 1)/Lip1p (Lag1p/Lac1p interacting protein) ceramide synthase. LAG1 and LAC1 are redundant but LIP1 is essential. Here we show that 4Δ (lag1Δlac1Δypc1Δydc1Δ) cells devoid of all known endogenous ceramide synthesis pathways are unviable but can be rescued by the expression of Lass5, a mouse LAG1 homologue. Ceramide synthase activity of 4Δ.Lass5 cells only utilizes C16 and C18 fatty acids and does not require the help of Lip1p, an essential cofactor of Lag1p/Lac1p. HPLC-electrospray ionization-MS/MS analysis demonstrated that in IPCs (inositolphosphorylceramides) of 4Δ.Lass5, the very long chain fatty acids (C26 and C24) account for <1% instead of the normal >97%. Notwithstanding, IPCs incorporated into glycosylphosphatidylinositol anchors of 4Δ.Lass5 show normal mobility on TLC and the ceramide- and raft-dependent traffic of Gas1p (glycophospholipid-anchored surface protein) from endoplasmic reticulum to Golgi remains almost normal. Moreover, the biosynthesis of C24:0 fatty acids remains essential. Thus, C24:0 and dihydrosphingosine are both necessary for survival of yeast cells even if they utilize C16 and C18 fatty acids for sphingolipid biosynthesis.
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January 2007
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Research Article|
December 11 2006
Yeast sphingolipids do not need to contain very long chain fatty acids
Vanessa Cerantola;
Vanessa Cerantola
*Department of Medicine/Biochemistry, University of Fribourg, Rue du Musée, CH-1700 Fribourg, Switzerland
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Christine Vionnet;
Christine Vionnet
*Department of Medicine/Biochemistry, University of Fribourg, Rue du Musée, CH-1700 Fribourg, Switzerland
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Olivier F. Aebischer;
Olivier F. Aebischer
†Department of Chemistry, University of Fribourg, Rue du Musée, CH-1700 Fribourg, Switzerland
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Titus Jenny;
Titus Jenny
†Department of Chemistry, University of Fribourg, Rue du Musée, CH-1700 Fribourg, Switzerland
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Jens Knudsen;
Jens Knudsen
‡University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark
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Andreas Conzelmann
Andreas Conzelmann
1
*Department of Medicine/Biochemistry, University of Fribourg, Rue du Musée, CH-1700 Fribourg, Switzerland
1To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
July 25 2006
Revision Received:
September 19 2006
Accepted:
September 20 2006
Accepted Manuscript online:
September 20 2006
Online ISSN: 1470-8728
Print ISSN: 0264-6021
The Biochemical Society, London
2007
Biochem J (2007) 401 (1): 205–216.
Article history
Received:
July 25 2006
Revision Received:
September 19 2006
Accepted:
September 20 2006
Accepted Manuscript online:
September 20 2006
Citation
Vanessa Cerantola, Christine Vionnet, Olivier F. Aebischer, Titus Jenny, Jens Knudsen, Andreas Conzelmann; Yeast sphingolipids do not need to contain very long chain fatty acids. Biochem J 1 January 2007; 401 (1): 205–216. doi: https://doi.org/10.1042/BJ20061128
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