Bacterial Hbs (haemoglobins), like VHb (Vitreoscilla sp. Hb), and flavoHbs (flavohaemoglobins), such as FHP (Ralstonia eutropha flavoHb), have different autoxidation and ligand-binding rates. To determine the influence of each domain of flavoHbs on ligand binding, we have studied the kinetic ligand-binding properties of oxygen, carbon monoxide and nitric oxide to the chimaeric proteins, FHPg (truncated form of FHP comprising the globin domain alone) and VHb-Red (fusion protein between VHb and the C-terminal reductase domain of FHP) and compared them with those of their natural counterparts, FHP and VHb. Moreover, we also analysed polarity and solvent accessibility to the haem pocket of these proteins. The rate constants for the engineered proteins, VHb-Red and FHPg, do not differ significantly from those of their natural counterparts, VHb and FHP respectively. Our results suggest that the globin domain structure controls the reactivity towards oxygen, carbon monoxide and nitric oxide. The presence or absence of a reductase domain does not affect the affinity to these ligands.
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September 12 2007
Analysis of the contribution of the globin and reductase domains to the ligand-binding properties of bacterial haemoglobins Available to Purchase
Judith Farrés;
Judith Farrés
*Institute of Biotechnology, ETH Zürich, CH 8093 Zürich, Switzerland
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Susanna Burckhardt-Herold;
Susanna Burckhardt-Herold
†Laboratory of Inorganic Chemistry, ETH Zürich, CH 8093 Zürich, Switzerland
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Jan Scherrer;
Jan Scherrer
*Institute of Biotechnology, ETH Zürich, CH 8093 Zürich, Switzerland
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Alexander D. Frey;
Alexander D. Frey
‡Institute of Microbiology, ETH Zürich, CH 8093 Zürich, Switzerland
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Pauli T. Kallio
Pauli T. Kallio
1
‡Institute of Microbiology, ETH Zürich, CH 8093 Zürich, Switzerland
1To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
May 18 2007
Revision Received:
June 25 2007
Accepted:
July 06 2007
Accepted Manuscript online:
July 06 2007
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2007 Biochemical Society
2007
Biochem J (2007) 407 (1): 15–22.
Article history
Received:
May 18 2007
Revision Received:
June 25 2007
Accepted:
July 06 2007
Accepted Manuscript online:
July 06 2007
Citation
Judith Farrés, Susanna Burckhardt-Herold, Jan Scherrer, Alexander D. Frey, Pauli T. Kallio; Analysis of the contribution of the globin and reductase domains to the ligand-binding properties of bacterial haemoglobins. Biochem J 1 October 2007; 407 (1): 15–22. doi: https://doi.org/10.1042/BJ20070668
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