ADAM (a disintegrin and metalloproteinase) 10 is a key member of the ADAM family of disintegrin and metalloproteinases which process membrane-associated proteins to soluble forms in a process known as ‘shedding’. Among the major targets of ADAM10 are Notch, EphrinA2 and CD44. In many cell-based studies of shedding, the activity of ADAM10 appears to overlap with that of ADAM17, which has a similar active-site topology relative to the other proteolytically active ADAMs. The tissue inhibitors of metalloproteinases, TIMPs, have proved useful in the study of ADAM function, since TIMP-1 inhibits ADAM10, but not ADAM17; however, both enzymes are inhibited by TIMP-3. In the present study, we show that, in comparison with ADAM17 and the MMPs (matrix metalloproteinases), the N-terminal domains of TIMPs alone are insufficient for the inhibition of ADAM10. This knowledge could form the basis for the design of directed inhibitors against different metalloproteinases.
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April 2008
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Research Article|
March 27 2008
The isolated N-terminal domains of TIMP-1 and TIMP-3 are insufficient for ADAM10 inhibition
Magdalini Rapti
;
Magdalini Rapti
1
*Department of Oncology, Cambridge University, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, U.K.
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Susan J. Atkinson
;
Susan J. Atkinson
1
*Department of Oncology, Cambridge University, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, U.K.
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Meng-Huee Lee
;
Meng-Huee Lee
*Department of Oncology, Cambridge University, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, U.K.
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Andrew Trim
;
Andrew Trim
†Department of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, U.K.
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Marcia Moss
;
Marcia Moss
‡Biozyme Inc., Apex, NC 7523, U.S.A.
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Gillian Murphy
Gillian Murphy
2
*Department of Oncology, Cambridge University, Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Cambridge CB2 0RE, U.K.
2To whom correspondence should be addressed (email gm290@cam.ac.uk).
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Biochem J (2008) 411 (2): 433–439.
Article history
Received:
October 17 2007
Revision Received:
December 11 2007
Accepted:
January 10 2008
Accepted Manuscript online:
January 10 2008
Citation
Magdalini Rapti, Susan J. Atkinson, Meng-Huee Lee, Andrew Trim, Marcia Moss, Gillian Murphy; The isolated N-terminal domains of TIMP-1 and TIMP-3 are insufficient for ADAM10 inhibition. Biochem J 15 April 2008; 411 (2): 433–439. doi: https://doi.org/10.1042/BJ20071430
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