Accurate chromosome segregation in mitosis is required to maintain genetic stability. hZwint-1 [human Zw10 (Zeste white 10)-interacting protein 1] is a kinetochore protein known to interact with the kinetochore checkpoint protein hZw10. hZw10, along with its partners Rod (Roughdeal) and hZwilch, form a complex which recruits dynein–dynactin and Mad1–Mad2 complexes to the kinetochore and are essential components of the mitotic checkpoint. hZwint-1 localizes to the kinetochore in prophase, before hZw10 localization, and remains at the kinetochore until anaphase, after hZw10 has dissociated. This difference in localization timing may reflect a role for hZwint-1 as a structural kinetochore protein. In addition to hZw10, we have found that hZwint-1 interacts with components of the conserved Ndc80 and Mis12 complexes in yeast two-hybrid and GST (glutathione transferase) pull-down assays. Furthermore, hZwint-1 was found to have stable FRAP (fluorescence recovery after photobleaching) dynamics similar to hHec1, hSpc24 and hMis12. As such, we proposed that hZwint-1 is a structural protein, part of the inner kinetochore scaffold and recruits hZw10 to the kinetochore. To test this, we performed mutagenesis-based domain mapping to determine which regions of hZwint-1 are necessary for kinetochore localization and which are required for interaction with hZw10. hZwint-1 localizes to the kinetochore through the N-terminal region and interacts with hZw10 through the C-terminal coiled-coil domain. The two domains are at opposite ends of the protein as expected for a protein that bridges the inner and outer kinetochore.
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Research Article|
April 27 2011
hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain
Larissa J. Vos;
Larissa J. Vos
*Department of Oncology, Faculty of Medicine and Dentistry, University of Alberta, 11560 University Avenue, Edmonton, Alberta, Canada, T6G 1Z2
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Jakub K. Famulski;
Jakub K. Famulski
1
*Department of Oncology, Faculty of Medicine and Dentistry, University of Alberta, 11560 University Avenue, Edmonton, Alberta, Canada, T6G 1Z2
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Gordon K. T. Chan
Gordon K. T. Chan
2
*Department of Oncology, Faculty of Medicine and Dentistry, University of Alberta, 11560 University Avenue, Edmonton, Alberta, Canada, T6G 1Z2
†School of Cancer, Engineering and Imaging Sciences, University of Alberta, Edmonton, Alberta, Canada, T6G 2B7
‡Experimental Oncology, Cross Cancer Institute, 11560 University Avenue, Edmonton, Alberta, Canada T6G 1Z2
2To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
January 20 2011
Revision Received:
February 16 2011
Accepted:
February 23 2011
Accepted Manuscript online:
February 23 2011
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem J (2011) 436 (1): 157–168.
Article history
Received:
January 20 2011
Revision Received:
February 16 2011
Accepted:
February 23 2011
Accepted Manuscript online:
February 23 2011
Citation
Larissa J. Vos, Jakub K. Famulski, Gordon K. T. Chan; hZwint-1 bridges the inner and outer kinetochore: identification of the kinetochore localization domain and the hZw10-interaction domain. Biochem J 15 May 2011; 436 (1): 157–168. doi: https://doi.org/10.1042/BJ20110137
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