AA (arachidonic acid), which possesses both neurotoxic and neurotrophic activities, has been implicated as a messenger in both physiological and pathophysiological processes. In the present study, we investigated the effects of both extracellular and intracellular application of AA on the activity of NaV (voltage-gated Na+ channels) in rat cerebellar GCs (granule cells). The extracellular application of AA inhibited the resultant INa (NaV current), wherein the current–voltage curve shifted to a negative voltage direction. Because this effect could be reproduced by treating the GCs with ETYA (eicosa-5,8,11,14-tetraynoic acid) or a membrane-impermeable analogue of AA, AA-CoA (arachidonoyl coenzyme A), we inferred that AA itself exerted the observed modulatory effects on INa. In contrast, intracellular AA significantly augmented the elicited INa peak when the same protocol that was used for extracellular AA was followed. The observed INa increase that was induced by intracellular AA was mimicked by the AA cyclo-oxygenase metabolite PGE2 (prostaglandin E2), but not by ETYA. Furthermore, cyclo-oxygenase inhibitors decreased INa and quenched AA-induced channel activation, indicating that the effect of intracellular AA on NaV was possibly mediated through AA metabolites. In addition, the PGE2-induced activation of INa was mimicked by cAMP and quenched by a PKA (protein kinase A) inhibitor, a Gs inhibitor and EP (E-series of prostaglandin) receptor antagonists. The results of the present study suggest that extracellular AA modulates NaV channel activity in rat cerebellar GCs without metabolic conversion, whereas intracellular AA augments the INa by PGE2-mediated activation of cAMP/PKA pathways. These observations may explain the dual character of AA in neuronal pathogenesis.
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Research Article|
July 27 2011
Arachidonic acid modulates Na+ currents by non-metabolic and metabolic pathways in rat cerebellar granule cells
Yan-Jia Fang;
Yan-Jia Fang
1Institute of Brain Science, School of Life Sciences and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200433, China
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Meng-Hua Zhou;
Meng-Hua Zhou
1Institute of Brain Science, School of Life Sciences and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200433, China
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Xiao-Fei Gao;
Xiao-Fei Gao
1Institute of Brain Science, School of Life Sciences and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200433, China
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Hua Gu;
Hua Gu
1Institute of Brain Science, School of Life Sciences and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200433, China
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Yan-Ai Mei
Yan-Ai Mei
1
1Institute of Brain Science, School of Life Sciences and State Key Laboratory of Medical Neurobiology, Fudan University, Shanghai 200433, China
1To whom correspondence should be addressed (email yamei@fudan.edu.cn).
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Biochem J (2011) 438 (1): 203–215.
Article history
Received:
March 29 2011
Accepted:
May 12 2011
Accepted Manuscript online:
May 12 2011
Citation
Yan-Jia Fang, Meng-Hua Zhou, Xiao-Fei Gao, Hua Gu, Yan-Ai Mei; Arachidonic acid modulates Na+ currents by non-metabolic and metabolic pathways in rat cerebellar granule cells. Biochem J 15 August 2011; 438 (1): 203–215. doi: https://doi.org/10.1042/BJ20110569
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