The factors that influence preadipocyte determination remain poorly understood. In the present paper, we report that CREBL2 [CREB (cAMP-response-element-binding protein)-like 2], a novel bZIP_1 protein, is up-regulated during MDI-induced preadipocyte differentiation. During both overexpression and under physiological conditions, CREBL2 interacted and was entirely co-localized with CREB. Overexpression of CREBL2 was sufficient to promote adipogenesis via up-regulating the expression of PPARγ (peroxisome-proliferator-activated receptor γ) and C/EBPα (CCAAT/enhancer-binding protein α) and accelerate lipogenesis accompanied with increased GLUT (glucose transporter) 1 and GLUT4. CREBL2 knockdown restrained adipogenic conversion and lipogenesis. Additionally, depletion of CREB could completely block the effects of overexpressed CREBL2, whereas an increase in CREB could not drive adipogenesis in the absence of CREBL2, indicating that the roles for CREBL2 on adipogenesis were CREB-dependent. Furthermore, siCREBL2 [siRNA (short interfering RNA) against CREBL2] could down-regulate CREB transcriptional activity and suppress CREB phosphorylation. CREB knockdown decreased the CREBL2 protein levels and vice versa. Collectively, the results of the present study indicate that CREBL2 plays a critical role in adipogenesis and lipogenesis via interaction with CREB.
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October 2011
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Research Article|
September 14 2011
CREBL2, interacting with CREB, induces adipogenesis in 3T3-L1 adipocytes
Xi Ma;
Xi Ma
*State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China
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Heyu Zhang;
Heyu Zhang
†Human Disease Genomics Center, Peking University, No. 38 Xueyuan Road, Beijing 100191, China
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Lan Yuan;
Lan Yuan
‡Peking University Medical and Health Analysis Center, Peking University, No. 38 Xueyuan Road, Beijing 100191, China
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Hao Jing;
Hao Jing
§College of Food Science and Nutritional Engineering, China Agricultural University, No. 17 Qinghua East Road, Beijing 100191, China
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Phil Thacker;
Phil Thacker
¶Department of Animal and Poultry, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, Canada
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Defa Li
Defa Li
1
*State Key Laboratory of Animal Nutrition, China Agricultural University, No. 2 Yuanmingyuan West Road, Beijing 100193, China
1To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
September 13 2010
Revision Received:
June 14 2011
Accepted:
June 28 2011
Accepted Manuscript online:
June 28 2011
Online ISSN: 1470-8728
Print ISSN: 0264-6021
© The Authors Journal compilation © 2011 Biochemical Society
2011
Biochem J (2011) 439 (1): 27–38.
Article history
Received:
September 13 2010
Revision Received:
June 14 2011
Accepted:
June 28 2011
Accepted Manuscript online:
June 28 2011
Citation
Xi Ma, Heyu Zhang, Lan Yuan, Hao Jing, Phil Thacker, Defa Li; CREBL2, interacting with CREB, induces adipogenesis in 3T3-L1 adipocytes. Biochem J 1 October 2011; 439 (1): 27–38. doi: https://doi.org/10.1042/BJ20101475
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